Arbovirus Diseases Branch, Division of Vector-borne and Infectious Diseases, Centers for Disease Control and Prevention, 3150 Rampart Road, Fort Collins, CO 80521, USA.
Mol Ecol Resour. 2009 Jan;9(1):4-18. doi: 10.1111/j.1755-0998.2008.02469.x.
DNA-based methods have greatly enhanced the sensitivity and specificity of hematophagous arthropod bloodmeal identification. A variety of methods have been applied to study the blood-feeding behaviour of mosquitoes, ticks, black flies and other blood-feeding arthropods as it relates to host-parasite interactions and pathogen transmission. Overviews of the molecular techniques used for bloodmeal identification, their advantages, disadvantages and applications are presented for DNA sequencing, group-specific polymerase chain reaction primers, restriction fragment length polymorphism, real-time polymerase chain reaction, heteroduplex analysis, reverse line-blot hybridization and DNA profiling. Technical challenges to bloodmeal identification including digestion and analysis of mixed bloodmeals are discussed. Analysis of bloodmeal identification results remains a challenge to the field, particularly with regard to incorporation of vertebrate census and ecology data. Future research directions for molecular analysis of arthropod bloodmeals are proposed.
基于 DNA 的方法极大地提高了噬血节肢动物血食鉴定的灵敏度和特异性。已经应用了多种方法来研究蚊子、蜱、黑蝇和其他噬血节肢动物的吸血行为，因为它与宿主-寄生虫相互作用和病原体传播有关。本文综述了用于血食鉴定的分子技术，包括 DNA 测序、组特异性聚合酶链反应引物、限制性片段长度多态性、实时聚合酶链反应、异源双链分析、反向线印迹杂交和 DNA 分析。讨论了血食鉴定的技术挑战，包括混合血食的消化和分析。对血食鉴定结果的分析仍然是该领域的一个挑战，特别是在结合脊椎动物普查和生态学数据方面。提出了未来对节肢动物血食进行分子分析的研究方向。
