Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543, Division of Zoology, Research Centre for Biology-LIPI, Jl. Raya Bogor Km 46, Cibinong-Bogor, 16911, Indonesia.
Mol Ecol Resour. 2009 Jan;9(1):37-40. doi: 10.1111/j.1755-0998.2008.02221.x. Epub 2008 Sep 15.
The All Birds Barcoding Initiative aims to assemble a DNA barcode database for all bird species, but the 648-bp 'barcoding' region of cytochrome c oxidase subunit I (COI) can be difficult to amplify in Southeast Asian perching birds (Aves: Passeriformes). Using COI sequences from complete mitochondrial genomes, we designed a primer pair that more reliably amplifies and sequences the COI barcoding region of Southeast Asian passerine birds. The 655-bp region amplified with these primers overlaps the COI region amplified with other barcoding primer pairs, enabling direct comparison of sequences with previously published DNA barcodes.
全鸟类 DNA 条形码计划旨在为所有鸟类物种建立一个 DNA 条形码数据库，但细胞色素 c 氧化酶亚基 I（COI）的 648bp“条形码”区域在东南亚雀形目鸟类（Aves: Passeriformes）中很难扩增。我们使用来自完整线粒体基因组的 COI 序列设计了一对引物，该引物对更可靠地扩增和测序了东南亚雀形目鸟类的 COI 条形码区域。用这些引物扩增的 655bp 区域与用其他条形码引物对扩增的 COI 区域重叠，从而能够与以前发表的 DNA 条形码进行直接比较。
