Naeem Zara, Masud Samrah, Hassan Shoaib, Naeem Muhammad
Institute of Pure and Applied Biology, Bahauddin Zakariya University, Multan, Pakistan.
Mitochondrial DNA B Resour. 2020 Jul 25;5(3):3000-3003. doi: 10.1080/23802359.2020.1768913.
DNA barcoding is a rapid, precise, and effective way of species identification. A short and standard target gene marker is used to create sequence profile of identified species. Specific tag or marker is used, which is derived from mitochondrial COI for identification. Effectiveness of this method axes the degree of divergence among species. Identification is necessary for their representation. In the present work, was used to study by using Cytochrome C Oxidase 1.The genetic distances were computed, and Neighbor Joining tree was constructed based on the Kimura 2 Parameter method. GenBank and BOLD revealed definitive identity matches. Conspecific and congeneric K2P nucleotide divergence was estimated. Evolutionary tree was analyzed clearly by relating their species to phylogenetic tree, as same as species were bunched under same tree node, while species were differently clustered under distinct nodes. These findings conclude that the gene sequence may serve as a milestone for identification and phylogenetic history of related species at molecular level.
DNA条形码是一种快速、精确且有效的物种鉴定方法。使用一个短的标准目标基因标记来创建已鉴定物种的序列图谱。使用源自线粒体细胞色素C氧化酶亚基I（COI）的特定标签或标记进行鉴定。该方法的有效性取决于物种之间的差异程度。为了呈现这些物种，鉴定是必要的。在本研究中，使用细胞色素C氧化酶亚基I进行研究。计算了遗传距离，并基于Kimura 2参数法构建了邻接树。GenBank和BOLD数据库显示了明确的身份匹配。估计了同种和同属的K2P核苷酸差异。通过将它们的物种与系统发育树相关联，清晰地分析了进化树，就像相同的物种聚集在同一树节点下，而不同的物种则聚集在不同的节点下。这些发现得出结论，该基因序列可作为在分子水平上鉴定相关物种及其系统发育历史的一个里程碑。
