开发并鉴定西藏特有的鱼类青海湖裸鲤(Glyptosternum maculatum)的 16 个多态性微卫星位点。

Development and characterization of 16 polymorphic microsatellite loci in the Tibet endemic fish Glyptosternum maculatum.

机构信息

College of Fisheries, Huazhong Agriculture University, Wuhan, Hubei 430070, China Key Laboratory of Freshwater Fish Germplasm Resources & Biotechnology of Chinese Ministry of Agriculture, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Jingzhou, Hubei 434000, China.

出版信息

Mol Ecol Resour. 2009 Jan;9(1):380-2. doi: 10.1111/j.1755-0998.2008.02459.x.

DOI:10.1111/j.1755-0998.2008.02459.x

PMID:21564656

Abstract

Sixteen novel polymorphic microsatellite loci were developed and characterized in Glyptosternum maculatum, an endemic fish inhabiting the Yarlung Zangbo River valley, Tibet, China. These loci were developed after the enrichment of microsatellite-containing genomic DNA fragments with a subtractive hybridization protocol. The polymorphism of these loci was analysed with 36 individuals representing a geographical population. The number of alleles found at these loci ranged from 3 to 10. The observed heterozygosity and expected heterozygosity ranged from 0 to 1 and from 0.31 to 0.75, respectively. These microsatellite loci will certainly facilitate the determination of the genetic structure of G. maculatum and the conservation of its genetic resource.

摘要

十六个新的多态微卫星基因座被开发并鉴定于中国西藏雅鲁藏布江流域特有的鱼类——斑重唇鱼。这些基因座是通过消减杂交技术富集含微卫星的基因组 DNA 片段而开发的。通过 36 个个体的地理种群分析了这些基因座的多态性。这些基因座的等位基因数范围为 3 到 10。观察到的杂合度和预期杂合度范围分别为 0 到 1 和 0.31 到 0.75。这些微卫星基因座将有助于确定斑重唇鱼的遗传结构和保护其遗传资源。