在夏威夷特有蜻蜓 Megalagrion xanthomelas（蜻蜓目：Coenagrionidae）中分离和特征化 16 个多态性微卫星位点。

Isolation and characterization of 16 polymorphic microsatellite loci in the endemic Hawaiian damselfly Megalagrion xanthomelas (Odonata: Coenagrionidae).

机构信息

Department of Biology, Bucknell University, Lewisburg, PA 17837, USA, Department of Ecology and Evolutionary Biology, Cornell University, Corson Hall, Ithaca, NY 14853, USA.

出版信息

Mol Ecol Resour. 2009 Jan;9(1):165-7. doi: 10.1111/j.1755-0998.2008.02337.x. Epub 2008 Oct 21.

DOI:10.1111/j.1755-0998.2008.02337.x

PMID:21564591

Abstract

Microsatellite loci have been isolated from two species of endemic Hawaiian damselflies, Megalagrion xanthomelas and M. eudytum, that are of conservation concern. Sixteen polymorphic loci were characterized in 32 M. xanthomelas from one population on Molokai and one on Hawaii Island. The total number of alleles per locus ranged from two to 16 and observed population heterozygosity ranged from 0.0 to 0.963. Eleven of these loci amplified successfully in M. eudytum as well. These loci will be used to further conservation efforts and infer genetic consequences of Pleistocene glaciations.

摘要

已经从两种具有保护意义的夏威夷特有蜻蜓物种——黄斑大蜻蜓（Megalagrion xanthomelas）和埃氏大蜻蜓（M. eudytum）中分离出微卫星位点。在莫洛凯岛和夏威夷岛上的一个种群中，对 32 只黄斑大蜻蜓的 16 个多态性位点进行了特征描述。每个位点的等位基因总数从 2 到 16 不等，观察到的种群杂合度从 0.0 到 0.963 不等。其中 11 个位点也成功地在埃氏大蜻蜓中扩增。这些位点将用于进一步的保护工作，并推断更新世冰川作用的遗传后果。