菜豆（Phaseolus vulgaris L.）基因组中13个新的多态性微卫星标记的分离与鉴定
Isolation and characterization of 13 new polymorphic microsatellite markers in the Phaseolus vulgaris L. (Common Bean) genome.
作者信息
Wang Aihua, Ding Yi, Hu Zhenhua, Lin Chufa, Wang Shuzhen, Wang Bingcai, Zhang Hongyuan, Zhou Guolin
机构信息
Hubei Provincial Engineering Research Center of Vegetable Seeds and Seedlings, Wuhan Vegetable Research Institute, Wuhan Academy of Agricultural Science & Technology, Wuhan 430345, China.
State Key Laboratory of Hybrid Rice, Department of Genetics, College of Life Sciences, Wuhan University, Wuhan 430072, China.
出版信息
Int J Mol Sci. 2012;13(9):11188-11193. doi: 10.3390/ijms130911188. Epub 2012 Sep 7.
In this study, 13 polymorphic microsatellite markers were isolated from the Phaseolus vulgaris L. (common bean) by using the Fast Isolation by AFLP of Sequence COntaining Repeats (FIASCO) protocol. These markers revealed two to seven alleles, with an average of 3.64 alleles per locus. The polymorphic information content (PIC) values ranged from 0.055 to 0.721 over 13 loci, with a mean value of 0.492, and 7 loci having PIC greater than 0.5. The expected heterozygosity (H(E)) and observed heterozygosity (H(O)) levels ranged from 0.057 to 0.814 and from 0.026 to 0.531, respectively. Cross-species amplification of the 13 prime pairs was performed in its related specie of Vigna unguiculata L. Seven out of all these markers showed cross-species transferability. These markers will be useful for future genetic diversity and population genetics studies for this agricultural specie and its related species.
在本研究中，通过使用含重复序列的AFLP快速分离（FIASCO）方案，从菜豆（Phaseolus vulgaris L.）中分离出13个多态性微卫星标记。这些标记揭示了2至7个等位基因，每个位点平均有3.64个等位基因。13个位点的多态信息含量（PIC）值范围为0.055至0.721，平均值为0.492，且有7个位点的PIC大于0.5。预期杂合度（H(E)）和观察到的杂合度（H(O)）水平分别为0.057至0.814和0.026至0.531。对13对引物进行了在其近缘物种豇豆（Vigna unguiculata L.）中的跨物种扩增。所有这些标记中有7个显示出跨物种转移性。这些标记将有助于该农业物种及其相关物种未来的遗传多样性和群体遗传学研究。
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