Wang Zhixin, Sun Yalin, Huang Xinfang, Li Feng, Liu Yuping, Zhu Honglian, Liu Zhengwei, Ke Weidong
Wuhan Academy of Agricultural Sciences, Wuhan, Hubei, China.
PeerJ. 2020 Dec 8;8:e10485. doi: 10.7717/peerj.10485. eCollection 2020.
Taro () is an important root and tuber crop cultivated worldwide. There are two main types of taro that vary in morphology of corm and cormel, 'dasheen' and 'eddoe'. The eddoe type () is predominantly distributed throughout China. Characterizing the genetic diversity present in the germplasm bank of taro is fundamental to better manage, conserve and utilize the genetic resources of this species. In this study, the genetic diversity of 234 taro accessions from 16 provinces of China was assessed using 132,869 single nucleotide polymorphism (SNP) markers identified by specific length amplified fragment-sequencing (SLAF-seq). Population structure and principal component analysis permitted the accessions to be categorized into eight groups. The genetic diversity and population differentiation of the eight groups were evaluated using the characterized SNPs. Analysis of molecular variance showed that the variation among eight inferred groups was higher than that within groups, while a relatively small variance was found among the two morphological types and 16 collection regions. Further, a core germplasm set comprising 41 taro accessions that maintained the genetic diversity of the entire collection was developed based on the genotype. This research is expected to be valuable for genetic characterization, germplasm conservation, and breeding of taro.
芋头是一种在全球范围内广泛种植的重要块根和块茎作物。芋头主要有两种类型，即“大魁芋”和“小魁芋”，它们的球茎和小鳞茎形态各异。小魁芋类型在中国分布较为广泛。了解芋头种质库中的遗传多样性，对于更好地管理、保护和利用该物种的遗传资源至关重要。在本研究中，利用通过特定长度扩增片段测序（SLAF-seq）鉴定的132,869个单核苷酸多态性（SNP）标记，对来自中国16个省份的234份芋头种质的遗传多样性进行了评估。群体结构和主成分分析将这些种质分为八组。利用鉴定出的SNP对这八组的遗传多样性和群体分化进行了评估。分子方差分析表明，推断出的八组之间的变异高于组内变异，而在两种形态类型和16个收集区域之间发现的变异相对较小。此外，基于基因型构建了一个包含41份芋头种质的核心种质库，该核心种质库保留了整个收集群体的遗传多样性。本研究有望为芋头的遗传特征分析、种质保护和育种提供有价值的参考。
