分离并鉴定 Chevrier's 田鼠（Apodemus chevrieri）的 14 个微卫星位点。

Isolation and characterization of 14 microsatellite loci for the Chevrier's field mouse (Apodemus chevrieri).

机构信息

Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Datun Road, Chaoyang District, Beijing 100101, China Sichuan Academy of Forestry, Chengdu, Sichuan 610066, China Institute of Rare Animals and Plants, China West Normal University, Nanchong, Sichuan 637002, China.

出版信息

Mol Ecol Resour. 2009 May;9(3):848-50. doi: 10.1111/j.1755-0998.2008.02302.x. Epub 2009 Feb 3.

DOI:10.1111/j.1755-0998.2008.02302.x

PMID:21564765

Abstract

We describe the isolation and characterization of 14 microsatellite loci in the Chevrier's field mouse from genomic DNA-enriched libraries. The 14 loci were highly polymorphic with numbers of alleles per locus in 24 individuals ranging from three to 13, observed heterozygosity from 0.381 to 0.867 and expected heterozygosity from 0.448 to 0.931. All loci followed Hardy-Weinberg expectations except for CFM3, CFM10 and CFM12 loci. No significant linkage association was found among all these loci. The 14 polymorphic microsatellite loci will be useful in studying phylogeography and population genetics of the Chevrier's field mouse.

摘要

我们从基因组 DNA 富集文库中描述了 Chevrier 田鼠的 14 个微卫星基因座的分离和特征。14 个基因座具有高度多态性，24 个个体的每个基因座的等位基因数从 3 到 13 不等，观察到的杂合度从 0.381 到 0.867，预期杂合度从 0.448 到 0.931。除了 CFM3、CFM10 和 CFM12 基因座外，所有基因座均遵循 Hardy-Weinberg 预期。所有这些基因座之间均未发现显著的连锁关联。这 14 个多态性微卫星基因座将有助于研究 Chevrier 田鼠的系统地理学和种群遗传学。