Departamento de Biología Vegetal y Ecología, Universidad de Sevilla, Profesor García González 2, 41012 Seville, Spain.
Mol Ecol Resour. 2008 Jul;8(4):904-6. doi: 10.1111/j.1755-0998.2008.02110.x.
We have developed a set of eight polymorphic nuclear microsatellite markers for the Mediterranean shrub Pistacia lentiscus by means of an enriched library method. Characterization for the eight loci was carried out on 42 individuals from two populations sampled in southern Spain. The overall number of alleles detected was 59, ranging from three to 13 per locus. Expected heterozygosity per locus and population ranged from 0.139 to 0.895. Two loci albeit only in one population (Seville) departed significantly from Hardy-Weinberg equilibrium expectations and no linkage disequilibrium between pairs of loci was detected. These markers will be used in studies of gene flow across a fragmented landscape.
我们通过富集文库法为地中海灌木黄连木开发了一套 8 个多态性核微卫星标记。在从西班牙南部采集的两个种群的 42 个个体中，对这 8 个位点进行了特征描述。共检测到 59 个等位基因，每个位点的范围从 3 到 13 个。每个位点和种群的预期杂合度范围从 0.139 到 0.895。尽管只有在一个种群（塞维利亚）中，有两个位点显著偏离哈迪-温伯格平衡预期，并且未检测到两个位点之间的连锁不平衡。这些标记将用于研究破碎景观中的基因流。
