中国龙虾微卫星位点的分离与特性分析。

Isolation and characterization of microsatellite loci in Palinurus elephas.

机构信息

Departament de Genètica, Facultat de Biologia, Universitat de Barcelona, Av. Diagonal 645, 08028 Barcelona, Spain.

出版信息

Mol Ecol Resour. 2008 Nov;8(6):1477-9. doi: 10.1111/j.1755-0998.2008.02230.x. Epub 2008 Aug 29.

DOI:10.1111/j.1755-0998.2008.02230.x

Abstract

The European spiny lobster (Palinurus elephas) mean annual catches have decreased alarmingly during recent decades along its entire distribution area due to stock over-exploitation, which makes it a primary target for conservation plans. A total of 164 microsatellite loci were isolated from a genomic library of P. elephas enriched for CA, GA, CAA and GATA repeats. A total of 15 polymorphic loci have been screened in 48 individuals. High numbers of alleles per locus (averaging 20 ± 10.5) and observed heterozygosity (averaging 0.789 ± 0.197) have been detected. None of the pairs of loci showed significant linkage disequilibrium. Two of the loci (Pael1 and Pael2) showed significant departure from Hardy-Weinberg equilibrium in Sagres, while Pael38 showed significant departure in Tunis. These highly polymorphic markers will be useful in determining the spatial patterns of genetic diversity between and within populations of Palinurus elephas.

摘要

近年来，由于过度捕捞，欧洲刺龙虾（Palinurus elephas）的总捕获量在其整个分布区域内惊人地减少，这使其成为保护计划的主要目标。从富含 CA、GA、CAA 和 GATA 重复序列的 Palinurus elephas 基因组文库中分离出了 164 个微卫星位点。在 48 个个体中筛选了 15 个多态性位点。每个位点的等位基因数量较多（平均 20±10.5），观察到的杂合度较高（平均 0.789±0.197）。没有发现一对位点存在显著的连锁不平衡。在 Sagres，有两个位点（Pael1 和 Pael2）偏离 Hardy-Weinberg 平衡，而在突尼斯，Pael38 位点偏离 Hardy-Weinberg 平衡。这些高度多态性的标记将有助于确定 Palinurus elephas 种群之间和种群内遗传多样性的空间模式。