在水稻稻瘟病菌（Magnaporthe oryzae）感染过程中表达的 CVNH-LysM 凝集素的结构功能分析。
Structure-function analysis of a CVNH-LysM lectin expressed during plant infection by the rice blast fungus Magnaporthe oryzae.
机构信息
Department of Structural Biology, University of Pittsburgh School of Medicine, Biomedical Science Tower 3, Pittsburgh, PA 15260, USA.
出版信息
Structure. 2011 May 11;19(5):662-74. doi: 10.1016/j.str.2011.03.004.
Abstract
The rice blast fungus Magnaporthe oryzae's genome encodes a hypothetical protein (MGG_03307) containing a type III CVNH lectin, in which a LysM domain is inserted between individual repeats of a single CVNH domain. At present, no structural or ligand binding data are available for any type III CVNH and functional studies in natural source organisms are scarce. Here, we report NMR solution structure and functional data on MGG_03307. The structure of the CVNH/LysM module revealed that intact and functionally competent CVNH and LysM domains are present. Using NMR titrations, carbohydrate specificities for both domains were determined, and it was found that each domain behaves as an isolated unit without any interdomain communication. Furthermore, live-cell imaging revealed a predominant localization of MGG_03307 within the appressorium, the specialized fungal cell for gaining entry into rice tissue. Our results suggest that MGG_03307 plays a role in the early stages of plant infection.
摘要
稻瘟病菌 Magnaporthe oryzae 的基因组编码了一个含有 III 型 CVNH 凝集素的假设蛋白（MGG_03307），其中单个 CVNH 结构域的重复之间插入了一个 LysM 结构域。目前，尚无任何 III 型 CVNH 的结构或配体结合数据，并且在天然来源的生物体中功能研究也很少。在这里，我们报告了关于 MGG_03307 的 NMR 溶液结构和功能数据。CVNH/LysM 模块的结构表明，完整且功能正常的 CVNH 和 LysM 结构域均存在。通过 NMR 滴定，确定了两个结构域的碳水化合物特异性，结果发现每个结构域都表现为一个独立的单元，没有任何结构域间的通信。此外，活细胞成像显示 MGG_03307 主要定位于附着胞内，附着胞是真菌细胞进入水稻组织的专门细胞。我们的结果表明，MGG_03307 在植物感染的早期阶段发挥作用。
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