State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Zhejiang Provincial Key Laboratory of Agricultural Microbiomics, Key Laboratory of Agricultural Microbiome (MARA), Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang, China.
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Zhejiang Provincial Key Laboratory of Agricultural Microbiomics, Key Laboratory of Agricultural Microbiome (MARA), Institute of Biotechnology, Zhejiang University, Hangzhou, Zhejiang, China.
Virulence. 2024 Dec;15(1):2403566. doi: 10.1080/21505594.2024.2403566. Epub 2024 Sep 16.
The filamentous fungus is widely recognized as a notorious plant pathogen responsible for causing rice blasts. With rapid advancements in molecular biology technologies, numerous regulatory mechanisms have been thoroughly investigated. However, most recent studies have predominantly focused on infection-related pathways or host defence mechanisms, which may be insufficient for developing novel structure-based prevention strategies. A substantial body of literature has utilized cryo-electron microscopy and X-ray diffraction to explore the relationships between functional components, shedding light on the identification of potential drug targets. Owing to the complexity of protein extraction and stochastic nature of crystallization, obtaining high-quality structures remains a significant challenge for the scientific community. Emerging computational tools such as AlphaFold for structural prediction, docking for interaction analysis, and molecular dynamics simulations to replicate in vivo conditions provide novel avenues for overcoming these challenges. In this review, we aim to consolidate the structural biological advancements in , drawing upon mature experimental experiences from other species such as and mammals. We aim to explore the potential of protein construction to address the invasion and proliferation of , with the goal of identifying new drug targets and designing small-molecule compounds to manage this disease.
丝状真菌被广泛认为是一种臭名昭著的植物病原体，可导致稻瘟病。随着分子生物学技术的快速发展，许多调控机制已被深入研究。然而，最近的大多数研究主要集中在感染相关途径或宿主防御机制上，这对于开发新型基于结构的预防策略可能还不够。大量文献利用低温电子显微镜和 X 射线衍射技术来探索功能成分之间的关系，揭示了潜在药物靶点的鉴定。由于蛋白质提取的复杂性和结晶的随机性，获得高质量的结构仍然是科学界的一个重大挑战。新兴的计算工具，如用于结构预测的 AlphaFold、用于相互作用分析的对接以及用于复制体内条件的分子动力学模拟，为克服这些挑战提供了新的途径。在这篇综述中，我们旨在整合丝状真菌的结构生物学进展，借鉴其他物种（如 和哺乳动物）成熟的实验经验。我们旨在探索蛋白质构建的潜力，以解决 的入侵和增殖问题，目的是确定新的药物靶点并设计小分子化合物来治疗这种疾病。
