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压力之下:稻瘟病菌对植物感染的生物学研究

Under pressure: investigating the biology of plant infection by Magnaporthe oryzae.

作者信息

Wilson Richard A, Talbot Nicholas J

机构信息

School of Biosciences, University of Exeter, Geoffrey Pope Building, Stocker Road, Exeter, United Kingdom.

出版信息

Nat Rev Microbiol. 2009 Mar;7(3):185-95. doi: 10.1038/nrmicro2032.

DOI:10.1038/nrmicro2032
PMID:19219052
Abstract

The filamentous fungus Magnaporthe oryzae causes rice blast, the most serious disease of cultivated rice. Cellular differentiation of M. oryzae forms an infection structure called the appressorium, which generates enormous cellular turgor that is sufficient to rupture the plant cuticle. Here, we show how functional genomics approaches are providing new insight into the genetic control of plant infection by M. oryzae. We also look ahead to the key questions that need to be addressed to provide a better understanding of the molecular processes that lead to plant disease and the prospects for sustainable control of rice blast.

摘要

丝状真菌稻瘟病菌可引发稻瘟病,这是栽培水稻最严重的病害。稻瘟病菌的细胞分化形成一种名为附着胞的侵染结构,该结构会产生巨大的细胞膨压,足以使植物角质层破裂。在此,我们展示了功能基因组学方法如何为深入了解稻瘟病菌对植物侵染的遗传控制提供新的见解。我们还展望了为更好地理解导致植物病害的分子过程以及稻瘟病可持续防治前景而需要解决的关键问题。

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1
Under pressure: investigating the biology of plant infection by Magnaporthe oryzae.压力之下:稻瘟病菌对植物感染的生物学研究
Nat Rev Microbiol. 2009 Mar;7(3):185-95. doi: 10.1038/nrmicro2032.
2
Investigating the cell and developmental biology of plant infection by the rice blast fungus Magnaporthe oryzae.研究稻瘟病菌(Magnaporthe oryzae)感染植物的细胞和发育生物学。
Fungal Genet Biol. 2021 Sep;154:103562. doi: 10.1016/j.fgb.2021.103562. Epub 2021 Apr 18.
3
Two independent S-phase checkpoints regulate appressorium-mediated plant infection by the rice blast fungus Magnaporthe oryzae.两个独立的S期检查点调控稻瘟病菌Magnaporthe oryzae通过附着胞介导的植物感染。
Proc Natl Acad Sci U S A. 2017 Jan 10;114(2):E237-E244. doi: 10.1073/pnas.1611307114. Epub 2016 Dec 27.
4
MoSnt2-dependent deacetylation of histone H3 mediates MoTor-dependent autophagy and plant infection by the rice blast fungus Magnaporthe oryzae.MoSnt2 依赖性组蛋白 H3 去乙酰化作用介导 MoTor 依赖性自噬,并促进稻瘟病菌对水稻的侵染。
Autophagy. 2018;14(9):1543-1561. doi: 10.1080/15548627.2018.1458171. Epub 2018 Aug 31.
5
Genome-wide transcriptional profiling of appressorium development by the rice blast fungus Magnaporthe oryzae.稻瘟病菌(Magnaporthe oryzae)附着胞发育的全基因组转录谱分析。
PLoS Pathog. 2012 Feb;8(2):e1002514. doi: 10.1371/journal.ppat.1002514. Epub 2012 Feb 9.
6
Evidence for a transketolase-mediated metabolic checkpoint governing biotrophic growth in rice cells by the blast fungus Magnaporthe oryzae.稻瘟病菌Magnaporthe oryzae通过转酮醇酶介导的代谢检查点调控水稻细胞中活体营养生长的证据。
PLoS Pathog. 2014 Sep 4;10(9):e1004354. doi: 10.1371/journal.ppat.1004354. eCollection 2014 Sep.
7
Rise of a Cereal Killer: The Biology of Magnaporthe oryzae Biotrophic Growth.稻瘟病菌的生物型营养生长的生物学机制
Trends Microbiol. 2018 Jul;26(7):582-597. doi: 10.1016/j.tim.2017.12.007. Epub 2018 Jan 24.
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The role of glycerol in the pathogenic lifestyle of the rice blast fungus Magnaporthe oryzae.甘油在稻瘟病菌Magnaporthe oryzae致病生活方式中的作用。
Environ Microbiol. 2017 Mar;19(3):1008-1016. doi: 10.1111/1462-2920.13688. Epub 2017 Mar 1.
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Phosphodiesterase MoPdeH targets MoMck1 of the conserved mitogen-activated protein (MAP) kinase signalling pathway to regulate cell wall integrity in rice blast fungus Magnaporthe oryzae.磷酸二酯酶MoPdeH作用于保守的丝裂原活化蛋白(MAP)激酶信号通路中的MoMck1,以调控稻瘟病菌稻瘟菌的细胞壁完整性。
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PKA activity is essential for relieving the suppression of hyphal growth and appressorium formation by MoSfl1 in Magnaporthe oryzae.蛋白激酶A(PKA)活性对于解除稻瘟病菌中MoSfl1对菌丝生长和附着胞形成的抑制作用至关重要。
PLoS Genet. 2017 Aug 14;13(8):e1006954. doi: 10.1371/journal.pgen.1006954. eCollection 2017 Aug.

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Effector-Triggered Immunity Is a Key Component of Nonhost Resistance in Nicotiana benthamiana against the Rice Blast Pathogen Magnaporthe oryzae.效应子触发免疫是本氏烟草对稻瘟病菌非寄主抗性的关键组成部分。
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MaMsb2, a signaling mucin, is involved in conidiation, stress tolerances, and virulence in the entomopathogenic fungus .

本文引用的文献

1
A multilocus gene genealogy concordant with host preference indicates segregation of a new species, Magnaporthe oryzae, from M. grisea.一个与宿主偏好一致的多基因系统发育表明,来自稻瘟病菌的一个新种,稻瘟霉,从灰霉菌中分离出来。
Mycologia. 2002 Jul-Aug;94(4):683-93. doi: 10.1080/15572536.2003.11833196.
2
Utility of cytoplasmic fluorescent proteins for live-cell imaging of Magnaporthe grisea in planta.细胞质荧光蛋白在活体植物中灰霉菌细胞成像的应用。
Mycologia. 2002 Mar-Apr;94(2):280-9.
3
Comparative genome analysis of filamentous fungi reveals gene family expansions associated with fungal pathogenesis.
MaMsb2是一种信号黏蛋白,参与昆虫病原真菌的分生孢子形成、胁迫耐受性和毒力。
Virulence. 2025 Dec;16(1):2541708. doi: 10.1080/21505594.2025.2541708. Epub 2025 Aug 2.
4
ChCpc1, a bZIP transcription factor, coordinates amino acid synthesis and autophagy and modulates conidiation and virulence in .ChCpc1是一种bZIP转录因子,它协调氨基酸合成与自噬,并调节分生孢子形成及致病性。
mBio. 2025 Aug 13;16(8):e0084525. doi: 10.1128/mbio.00845-25. Epub 2025 Jul 21.
5
The Mgv1-Rlm1 axis orchestrates SAGA and SWI/SNF complexes at target promoters.Mgv1-Rlm1轴在靶基因启动子处协调SAGA和SWI/SNF复合物。
Nucleic Acids Res. 2025 Jul 8;53(13). doi: 10.1093/nar/gkaf653.
6
Defending rice crop from blast disease in the context of climate change for food security in Nepal.在气候变化背景下保护尼泊尔水稻作物免受稻瘟病侵害以保障粮食安全。
Front Plant Sci. 2025 Jun 25;16:1511945. doi: 10.3389/fpls.2025.1511945. eCollection 2025.
7
H2S-mediated protein S-sulfhydration modulates infectivity and autophagy in the rice blast fungus.硫化氢介导的蛋白质S-硫氢化作用调节稻瘟病菌的感染性和自噬作用。
Nat Commun. 2025 Jul 5;16(1):6222. doi: 10.1038/s41467-025-61582-8.
8
Decapeptide Inducer Promotes the Conidiation of Phytopathogenic via the Mps1 MAPK Signaling Pathway.十肽诱导剂通过Mps1丝裂原活化蛋白激酶信号通路促进植物病原菌的分生孢子形成。
Int J Mol Sci. 2025 Jun 19;26(12):5880. doi: 10.3390/ijms26125880.
9
Molecular arrangements that accompany binding of rice xylanase inhibitor protein OsXIP and the Rhizopus oryzae GH11 xylanase RXyn2.水稻木聚糖酶抑制蛋白OsXIP与米根霉GH11木聚糖酶RXyn2结合时的分子排列。
J Biol Chem. 2025 Jun 16;301(8):110385. doi: 10.1016/j.jbc.2025.110385.
10
Molecular Insights into Rice Immunity: Unveiling Mechanisms and Innovative Approaches to Combat Major Pathogens.水稻免疫的分子见解:揭示对抗主要病原体的机制和创新方法。
Plants (Basel). 2025 Jun 1;14(11):1694. doi: 10.3390/plants14111694.
丝状真菌的比较基因组分析揭示了与真菌致病性相关的基因家族扩张。
PLoS One. 2008 Jun 4;3(6):e2300. doi: 10.1371/journal.pone.0002300.
4
Mapping and quantifying mammalian transcriptomes by RNA-Seq.通过RNA测序对哺乳动物转录组进行定位和定量分析。
Nat Methods. 2008 Jul;5(7):621-8. doi: 10.1038/nmeth.1226. Epub 2008 May 30.
5
Transcriptome analysis reveals new insight into appressorium formation and function in the rice blast fungus Magnaporthe oryzae.转录组分析揭示了对稻瘟病菌稻瘟菌附着胞形成和功能的新见解。
Genome Biol. 2008;9(5):R85. doi: 10.1186/gb-2008-9-5-r85. Epub 2008 May 20.
6
Systematic functional analysis of calcium-signalling proteins in the genome of the rice-blast fungus, Magnaporthe oryzae, using a high-throughput RNA-silencing system.利用高通量RNA沉默系统对稻瘟病菌Magnaporthe oryzae基因组中的钙信号蛋白进行系统功能分析。
Mol Microbiol. 2008 Jun;68(6):1348-65. doi: 10.1111/j.1365-2958.2008.06242.x. Epub 2008 Apr 21.
7
A putative MAP kinase kinase kinase, MCK1, is required for cell wall integrity and pathogenicity of the rice blast fungus, Magnaporthe oryzae.一种假定的丝裂原活化蛋白激酶激酶激酶MCK1,是稻瘟病菌细胞壁完整性和致病性所必需的。
Mol Plant Microbe Interact. 2008 May;21(5):525-34. doi: 10.1094/MPMI-21-5-0525.
8
MADS-box transcription factor mig1 is required for infectious growth in Magnaporthe grisea.MADS盒转录因子mig1是稻瘟病菌侵染性生长所必需的。
Eukaryot Cell. 2008 May;7(5):791-9. doi: 10.1128/EC.00009-08. Epub 2008 Mar 14.
9
RXLR effector reservoir in two Phytophthora species is dominated by a single rapidly evolving superfamily with more than 700 members.两种疫霉属物种中的RXLR效应子库由一个拥有700多个成员的快速进化的单一超家族主导。
Proc Natl Acad Sci U S A. 2008 Mar 25;105(12):4874-9. doi: 10.1073/pnas.0709303105. Epub 2008 Mar 14.
10
Rice blast infection of Brachypodium distachyon as a model system to study dynamic host/pathogen interactions.以二穗短柄草的稻瘟病感染作为研究宿主/病原体动态相互作用的模型系统。
Nat Protoc. 2008;3(3):435-45. doi: 10.1038/nprot.2007.499.