Yoshino Yoko, Nakagawa Yoichi
Department of Nutrition and Dietetics, Kamakura Women's University, 6-1-3 Ohfuna, Kamakura, Kanagawa 247-8512, Japan.
Open Dent J. 2011 Mar 29;5:48-51. doi: 10.2174/1874210601105010048.
We previously reported that physical exercise under food restriction induced alterations in saliva secretion, including a decrease in salivary kallikrein activity, while exercise training alone did not affect the overall saliva content. The objective of the present study was to examine the involvement of oxidative stress in alterations of salivary secretion due to physical exercise under food restriction.
Male ICR mice at 32 weeks of age weighing from 39 to 50 g were divided into three groups: an exercise group with food restriction (EXP), an exercise group without food restriction (EXA) and a control group (CTL). The EXP group was fed the same amount of diet as the CTL group (pair-feeding). The EXP and EXA groups had access to a "voluntary running wheel" for exercise. The pilocarpine-stimulated whole saliva was collected from the oral cavity by micro-pipette over 15 min for 12 weeks after the beginning of the experiment. The salivary and serum 8-Hydroxy-2'-deoxyguanosine (8-OHdG) were determined.
The salivary 8-OHdG levels in the EXP group were significantly higher than in the CTL and EXA groups (p<0.01).
The alterations of salivary secretion in mice undergoing chronic exercise training under a food-restricted diet may occur as a result of oxidative stress.
我们之前报道过，在食物限制条件下进行体育锻炼会引起唾液分泌的改变，包括唾液激肽释放酶活性降低，而单独的运动训练并未影响唾液的总体含量。本研究的目的是探究氧化应激在食物限制条件下体育锻炼引起的唾液分泌改变中的作用。
将32周龄、体重39至50克的雄性ICR小鼠分为三组：食物限制运动组（EXP）、无食物限制运动组（EXA）和对照组（CTL）。EXP组给予与CTL组相同量的饮食（配对喂养）。EXP组和EXA组可使用“自愿跑步轮”进行锻炼。实验开始12周后，用微量移液器在15分钟内从口腔收集毛果芸香碱刺激后的全唾液。测定唾液和血清中的8-羟基-2'-脱氧鸟苷（8-OHdG）。
EXP组的唾液8-OHdG水平显著高于CTL组和EXA组（p<0.01）。
在食物限制饮食条件下进行慢性运动训练的小鼠唾液分泌的改变可能是氧化应激的结果。
