Department of Nutrition and Dietics, Kamakura Women's University, 6-1-3 Ohfuna, Kamakura, Kanagawa 247-8512, Japan.
Arch Oral Biol. 2009 Nov;54(11):977-85. doi: 10.1016/j.archoralbio.2009.07.012. Epub 2009 Sep 6.
The purpose of the study was to assess the alterations of the autonomic nervous system activities caused by physical exercise training under food restriction using a running wheel in mice based on an analysis of saliva.
Male ICR mice, weighing 39-50 g, at 32 weeks of age were divided into three groups; an exercise with food restriction (EXP), an exercise without food restriction (EXA) and a control (CTL). The EXP group was fed the same amount of diet as the CTL group (pair-feeding). The EXP and EXA groups used a "voluntary running wheel" for exercise. The pilocarpine stimulated whole saliva was collected from the oral cavity by micropipette over 15 min 4, 8 and 12 weeks after the beginning of the experiment. The salivary flow rate, protein concentration and amylase and kallikrein activities were determined, since amylase and kallikrein release have been shown to be evoked by beta- and alpha-adrenergic receptor stimulation, respectively.
There was no significant difference in the sera corticosterone levels among the three experimental groups. The flow rate of saliva per total salivary gland weight in the EXP was significantly lower than that in the CTL and the EXA groups. The total protein secretion and kallikrein activity decreased by 20-30% in the EXP mice between 4 and 12 weeks after the starting of experiments in comparison to the CTL mice, thus suggesting that signal transduction in the alpha-sympathetic nervous system was downregulated. There was no significant difference in the amylase activity between the EXP and the CTL groups.
Physical exercise under diet control induced alterations in saliva secretion, while exercise training alone did not affect the content of saliva. The results demonstrate the availability of saliva and suggest that the present experimental situation is a suitable experimental model for sports activity performed under diet control.
本研究旨在通过分析唾液，评估限制食物摄入的情况下，运动训练对小鼠自主神经系统活动的影响。
32 周龄雄性 ICR 小鼠，体重 39-50g，分为三组：运动限制饮食组（EXP）、运动非限制饮食组（EXA）和对照组（CTL）。EXP 组的饮食量与 CTL 组相同（等量喂养）。EXP 和 EXA 组使用“自愿跑轮”进行运动。用微量移液器从口腔采集毛果芸香碱刺激的全唾液，实验开始后 4、8 和 12 周采集 15 分钟。测定唾液流率、蛋白浓度及淀粉酶和激肽释放酶活性，因为淀粉酶和激肽释放酶的释放分别被证实是由β-和α-肾上腺素能受体刺激引起的。
三组实验动物的血清皮质酮水平无显著性差异。EXP 组的总唾液腺重量的唾液流量明显低于 CTL 和 EXA 组。在实验开始后 4-12 周期间，EXP 组的总蛋白分泌和激肽释放酶活性下降了 20-30%，这表明α-交感神经信号转导受到了下调。EXP 组和 CTL 组的淀粉酶活性无显著性差异。
饮食控制下的运动导致唾液分泌发生改变，而单独运动训练不会影响唾液量。结果表明，该实验模型可用于研究饮食控制下的运动活动。
