Nanjing Medical University, Department of Pathology, Nanjing 210029, PR China.
Oncol Rep. 2011 Aug;26(2):511-7. doi: 10.3892/or.2011.1295. Epub 2011 May 4.
Hepatoma-derived growth factor (HDGF) has been implicated in the growth and metastasis of various types of human cancer, but the role of HDGF expression in prostate cancer or breast cancer has not been documented. To assess the role of HDGF in the proliferation, migration and invasion by prostate and breast cancer cells, HDGF expression in DU145 and MCF7 cells was knocked down using siRNA, and the effect of such knockdown was assessed by MTS and [3H]-thymidine incorporation Transwell assays. Moreover, we identified differentially expressed genes that might mediate the HDGF-induced cellular effects. Our results demonstrate that down-regulation of HDGF expression significantly reduces the proliferation of both DU145 and MCF7 cells. However, down-regulation of HDGF expression in DU145 inhibited cell migration and invasion, but in MCF7 cells it stimulated cell migration and invasion. This differential effect might result from the differential induction of PIK3R1 or SERPINE1 in the two cell lines upon HDGF-siRNA treatment. In conclusion, HDGF may participate in the pathogenesis of prostate and breast cancer by promoting cell growth and it may be a therapeutic target for these cancers.
肝癌衍生生长因子（HDGF）已被牵涉到多种人类癌症的生长和转移中，但 HDGF 表达在前列腺癌或乳腺癌中的作用尚未有文献记载。为了评估 HDGF 在前列腺癌和乳腺癌细胞增殖、迁移和侵袭中的作用，我们使用 siRNA 敲低了 DU145 和 MCF7 细胞中的 HDGF 表达，并通过 MTS 和 [3H]-胸苷掺入 Transwell 分析评估了这种敲低的效果。此外，我们鉴定了可能介导 HDGF 诱导的细胞效应的差异表达基因。我们的结果表明，下调 HDGF 表达显著降低了 DU145 和 MCF7 细胞的增殖。然而，下调 HDGF 表达在 DU145 中抑制了细胞迁移和侵袭，但在 MCF7 细胞中刺激了细胞迁移和侵袭。这种差异效应可能是由于 HDGF-siRNA 处理后两种细胞系中 PIK3R1 或 SERPINE1 的差异诱导所致。总之，HDGF 可能通过促进细胞生长参与前列腺癌和乳腺癌的发病机制，因此可能成为这些癌症的治疗靶点。
