González-Salgado Amaia, González-Jaén Teresa, Vázquez Covadonga, Patiño Belén
Department of Microbiology III, Universidad Complutense de Madrid, Madrid, Spain.
Methods Mol Biol. 2011;739:211-6. doi: 10.1007/978-1-61779-102-4_19.
Aspergillus flavus is an important fungal species that frequently contaminates food commodities with diverse toxins, with aflatoxins being the most relevant in food safety. In addition, this is one of the major pathogenic Aspergillus species. In this work, specific PCR-based protocol for this species is described which allows the discrimination of other closely related species from the Aspergillus section Flavi, particularly Aspergillus parasiticus. The specific primers were designed on the multicopy internal transcribed region of the rDNA unit (ITS1-5.8S-ITS2 rDNA).
黄曲霉是一种重要的真菌物种，经常用多种毒素污染食品，其中黄曲霉毒素在食品安全方面最为相关。此外，它是主要的致病性曲霉菌种之一。在这项工作中，描述了针对该物种的基于PCR的特定方案，该方案能够将黄曲霉属中其他密切相关的物种，特别是寄生曲霉区分开来。特异性引物是基于rDNA单元的多拷贝内部转录区域（ITS1-5.8S-ITS2 rDNA）设计的。
