González-Salgado Amaia, González-Jaén Teresa, Vázquez Covadonga, Patiño Belén
Department of Genetics, Universidad Complutense de Madrid, Madrid 28040, Spain.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2008 Jun;25(6):758-64. doi: 10.1080/02652030701765715.
Aspergillus flavus is frequently found in food, producing a wide variety of toxins, aflatoxins being the most relevant in food safety. A specific PCR-based protocol for this species is described which allowed discrimination from other closely related species having different profiles of secondary metabolites from the Aspergillus Section Flavi, particularly A. parasiticus. The specific primers were designed on the multi-copy internal transcribed region of the rDNA unit (ITS1-5.8S-ITS2 rDNA) and were tested in a wide sample of related species and other fungal species commonly found in food. The PCR assay was coupled with a fungal enrichment and a DNA extraction method for wheat flour to enhance the sensitivity of the diagnostic protocol. The results indicated that the critical PCR amplification product was clearly observed for wheat flour contaminated by 10(2) spores after 16 h of incubation.
黄曲霉常见于食物中，会产生多种毒素，其中黄曲霉毒素与食品安全最为相关。本文描述了一种基于PCR的针对该菌种的方法，该方法能够区分黄曲霉属中其他产生不同次生代谢产物谱的近缘菌种，尤其是寄生曲霉。特异性引物是基于核糖体DNA单元（ITS1-5.8S-ITS2 rDNA）的多拷贝内部转录区域设计的，并在一系列相关菌种以及食物中常见的其他真菌菌种样本中进行了测试。PCR检测与一种用于小麦粉的真菌富集和DNA提取方法相结合，以提高诊断方法的灵敏度。结果表明，经16小时培养后，对于被10²个孢子污染的小麦粉，能清晰观察到关键的PCR扩增产物。
