Ji Lei, Wu Xiao-Fang, Xu De-Shun, Gong Li-Ming
Huzhou Center for Disease Control and Prevention, Huzhou 313000, China.
Bing Du Xue Bao. 2011 Sep;27(5):469-74.
To study the molecular characteristics of Noroviruses causing outbreaks of acute gastroenteritis in Huzhou. During April 2008 and February 2009, fecal specimens of patients collected from 2 outbreaks of acute gastroenteritis were tested for Norovirus by real-time RT-PCR. Partial sequence of RNA dependent RNA polymerase(RdRp) of the positive samples were amplified by RT-PCR, the PCR products were then purified, sequenced and phylogenetic analysis was conducted. Both genogroup II (GII) and genogroup I (GI) noroviruses were detected in 2 outbreaks. Phylogenetic analysis revealed that two of the GI norovirus strains isolated from 2008 belonged to genotype GI/2 and one of the GI Norovirus strain isolated from 2009 belonged to genotype GI/3. The other GIIú norovirus strains isolated from 2009 had high nucleotide identity with GIIb genotype that had been reported frequently in European countries during 2000 and 2001 and in Asian countries recently. These results suggested that the epidemic strains of norovirus isolated in Huzhou had a high degree of genetic diversity and prevalent genotypes at different times were also different. To our knowledge this is the first report of detecting GIIb variant in outbreaks of acute gastroenteritis in China.
研究引起湖州市急性胃肠炎暴发的诺如病毒的分子特征。在2008年4月至2009年2月期间，对从2起急性胃肠炎暴发中收集的患者粪便标本进行实时逆转录聚合酶链反应（RT-PCR）检测诺如病毒。通过RT-PCR扩增阳性样本的RNA依赖RNA聚合酶（RdRp）的部分序列，然后对PCR产物进行纯化、测序并进行系统发育分析。在2起暴发中均检测到II型（GII）和I型（GI）诺如病毒。系统发育分析显示，2008年分离的2株GI诺如病毒株属于GI/2基因型，2009年分离的1株GI诺如病毒株属于GI/3基因型。2009年分离的其他GII型诺如病毒株与2000年至2001年在欧洲国家以及最近在亚洲国家频繁报道的GIIb基因型具有高度核苷酸同一性。这些结果表明，湖州市分离的诺如病毒流行株具有高度的遗传多样性，不同时期的流行基因型也不同。据我们所知，这是中国首次在急性胃肠炎暴发中检测到GIIb变异株的报告。
