Université de Lille Nord de France, 59000 Lille, France.
Acta Biomater. 2011 Aug;7(8):3141-9. doi: 10.1016/j.actbio.2011.04.020. Epub 2011 Apr 27.
The aim of this work was to develop a polypropylene (PP) artificial abdominal wall implant for the prolonged release of ciprofloxacin (CFX). This sustained release effect was obtained by functionalization of the textile mesh with citric acid and hydroxypropyl-γ-cyclodextrin (HPγCD) or maltodextrin (MD). In both cases the textile finishing reaction yielded a cyclo- or malto-dextrin crosslinked polymer coating the fibers. The modified supports were characterized by thermogravimetric analysis (TGA), differential scanning calorimetry and scanning electron microscopy. The sorption capacities and the kinetics of CFX release were studied by batch tests coupled with spectrophotometric assays. Microbiological assays were carried out on Staphylococcus aureus, Staphylococcus epidermidis and Escherichia coli, while proliferation and viability tests used fibroblasts. The main results were as follows. (i) Due to the differences between the range of temperature of thermal degradation of the (cyclo)dextrins polymers and of the PP fibers TGA was a reliable method for quantifying the degree of functionalization of the textiles. (ii) Both modified supports showed improved sorption/desorption capacities for CFX, compared with the virgin mesh. The HPγCD-finished support showed an increased sorption capacity and a lower release rate of CFX compared with the MD modified support. (iii) Microbiological assays confirmed the latter result, with greater sustained antibacterial activity of the HPγCD treated support. These experiments have demonstrated the role of the cyclodextrin cavity in interactions with CFX: the antibiotic was not only adsorbed via hydrogen and acid-base interactions with the polyCTR-HPγCD network, but also via host-guest complexation. (iv) Biological tests revealed a slight decrease in fibroblast proliferation after 6 days on the modified supports, but cell viability tests showed that this was not due to toxicity of the (cyclo)dextrin polymer coatings.
这项工作的目的是开发一种用于延长释放环丙沙星（CFX）的聚丙烯（PP）人工腹壁植入物。通过柠檬酸和羟丙基-γ-环糊精（HPγCD）或麦芽糊精（MD）对纺织网进行功能化，获得了这种持续释放效果。在这两种情况下，纺织整理反应都会产生交联聚合物的环糊精或麦芽糊精涂层纤维。改性后的支撑物通过热重分析（TGA）、差示扫描量热法和扫描电子显微镜进行了表征。通过批量试验和分光光度测定研究了 CFX 释放的吸附能力和动力学。对金黄色葡萄球菌、表皮葡萄球菌和大肠杆菌进行了微生物学试验，而对成纤维细胞进行了增殖和活力试验。主要结果如下。（i）由于（环）糊精聚合物和 PP 纤维的热降解温度范围不同，TGA 是定量测定纺织品官能化程度的可靠方法。（ii）与原始网相比，两种改性支撑物对 CFX 的吸附/解吸能力均有所提高。与 MD 改性支撑物相比，HPγCD 处理的支撑物具有更高的 CFX 吸附能力和更低的释放率。（iii）微生物学试验证实了后者的结果，即 HPγCD 处理的支撑物具有更强的持续抗菌活性。这些实验证明了环糊精空腔在与 CFX 相互作用中的作用：抗生素不仅通过与聚 CTL-HPγCD 网络的氢键和酸碱相互作用被吸附，而且还通过主客体络合作用被吸附。（iv）生物试验显示，在改性支撑物上培养 6 天后，成纤维细胞的增殖略有下降，但细胞活力试验表明，这不是由于（环）糊精聚合物涂层的毒性所致。
