Anses, French Agency for Food, Environmental and Occupational Health Safety, BP53, 22440 Ploufragan, France.
Food Microbiol. 2011 Aug;28(5):862-8. doi: 10.1016/j.fm.2010.11.003. Epub 2010 Nov 16.
In order to estimate the prevalence of Campylobacter spp. and Salmonella spp. on broiler chicken carcasses and the prevalence of Campylobacter spp. in caeca, 58 French slaughterhouses were investigated in 2008. Enumeration of Campylobacter spp. was also performed in order to study the relation between caeca and carcass contamination. A pool of 10 caeca and one carcass were collected from 425 different batches over a 12-month period in 2008. Salmonella was isolated on 32 carcasses leading to a prevalence of 7.5% (5.0-10.0). The prevalence of Campylobacter was 77.2% (73.2-81.2) in caeca and 87.5% (84.4-90.7) on carcasses. No significant correlation was found between Campylobacter and Salmonella. Positive values of Campylobacter were normally distributed and the average level was 8.05 log(10) cfu/g (7.94-8.16) in caeca and 2.39 cfu/g (2.30-2.48) on carcasses. A positive correlation (r = 0.59) was found between the mean of Campylobacter in caeca and on carcasses (p < 0.001). Thus, carcasses from batches with Campylobacter-positive caeca had significantly (p < 0.001) higher numbers of Campylobacter per gram than batches with negative caeca. These results show that Campylobacter can be present in both matrices and reduction in caeca could be a possible way to reduce the amount of bacteria on carcasses. Of the 2504 identifications performed, 3 species of Campylobacter (Campylobacter jejuni, Campylobacter coli and Campylobacter lari) were identified. The main species recovered were C. jejuni and C. coli, which were isolated in 55.3% and 44.5% of positive samples, respectively. These two species were equally represented in caeca but C. jejuni was the most frequently isolated on carcasses with 57.1% and 42.5% of positive carcasses for C. jejuni and C. coli, respectively. This study underlines that target a reduction of Campylobacter on final products requires a decrease of contamination in caeca.
为了评估 2008 年法国 58 家屠宰场肉鸡胴体中弯曲杆菌和沙门氏菌的流行情况，以及盲肠中弯曲杆菌的流行情况，对其进行了调查。为了研究盲肠和胴体污染之间的关系，还对弯曲杆菌进行了计数。2008 年 12 个月期间，从 425 个不同批次中采集了 10 个盲肠和 1 个胴体的样本。沙门氏菌在 32 个胴体上被分离出来，流行率为 7.5%（[5.0-10.0]（95%CI））。盲肠中弯曲杆菌的流行率为 77.2%（[73.2-81.2]（95%CI）），胴体中弯曲杆菌的流行率为 87.5%（[84.4-90.7]（95%CI））。未发现弯曲杆菌和沙门氏菌之间存在显著相关性。弯曲杆菌的阳性值呈正态分布，盲肠中的平均水平为 8.05 log(10) cfu/g（[7.94-8.16]（95%CI）），胴体中的平均水平为 2.39 cfu/g（[2.30-2.48]（95%CI））。盲肠和胴体中弯曲杆菌的平均值之间存在正相关（r=0.59）（p<0.001）。因此，来自盲肠中弯曲杆菌阳性批次的胴体每克中弯曲杆菌的数量明显（p<0.001）高于盲肠中弯曲杆菌阴性批次的胴体。这些结果表明，弯曲杆菌可以存在于两种基质中，减少盲肠中的细菌数量可能是减少胴体中细菌数量的一种方法。在进行的 2504 次鉴定中，鉴定出了 3 种弯曲杆菌（空肠弯曲杆菌、结肠弯曲杆菌和鸭弯曲杆菌）。主要分离到的物种是空肠弯曲杆菌和结肠弯曲杆菌，分别占阳性样本的 55.3%和 44.5%。这两种物种在盲肠中同样存在，但空肠弯曲杆菌在胴体中分离得最多，阳性胴体中分别有 57.1%和 42.5%为空肠弯曲杆菌和结肠弯曲杆菌。这项研究强调，要降低最终产品中弯曲杆菌的含量，就必须减少盲肠中的污染。
