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DNA初免/蛋白加强疫苗接种对鸡免疫反应和肠道微生物群的影响。

Impact of DNA Prime/Protein Boost Vaccination against on Immune Responses and Gut Microbiota in Chickens.

作者信息

Gloanec Noémie, Dory Daniel, Quesne Ségolène, Béven Véronique, Poezevara Typhaine, Keita Alassane, Chemaly Marianne, Guyard-Nicodème Muriel

机构信息

GVB-Viral Genetics and Biosafety Unit, French Agency for Food, Environmental and Occupational Health and Safety (ANSES), 22440 Ploufragan, France.

HQPAP-Unit of Hygiene and Quality of Poultry and Pork Products, French Agency for Food, Environmental and Occupational Health and Safety (ANSES), 22440 Ploufragan, France.

出版信息

Vaccines (Basel). 2022 Jun 20;10(6):981. doi: 10.3390/vaccines10060981.

DOI:10.3390/vaccines10060981
PMID:35746589
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9231206/
Abstract

is reported to be the leading zoonosis in Europe, and poultry is the main reservoir of Despite all the efforts made, there is still no efficient vaccine to fight this bacterium directly in poultry. Recent studies have reported interactions between the chicken immune system and gut microbiota in response to colonisation. The present study was designed to analyse in more depth the immune responses and caecal microbiota following vaccination with a DNA prime/protein boost flagellin-based vaccine that induces some protection in specific-pathogen-free White Leghorn chickens, as shown previously. These data may help to improve future vaccination protocols against in poultry. Here a vaccinated and a placebo group were challenged by at the age of 19 days. A partial reduction in loads was observed in the vaccinated group. This was accompanied by the production of specific systemic and mucosal antibodies. Transient relatively higher levels of Interleukin-10 and antimicrobial peptide avian β-defensin 10 gene expressions were observed in the vaccinated and placebo groups respectively. The analysis of caecal microbiota revealed the vaccination's impact on its structure and composition. Specifically, levels of operational taxonomic units classified as and increased on day 40.

摘要

据报道，[疾病名称]是欧洲主要的人畜共患病，家禽是其主要宿主。尽管已做出诸多努力，但仍没有能在家禽中直接对抗这种细菌的有效疫苗。最近的研究报道了鸡免疫系统与肠道微生物群在应对[细菌名称]定植时的相互作用。本研究旨在更深入地分析用DNA初免/蛋白加强鞭毛蛋白疫苗接种后，特定病原体-free白来航鸡的免疫反应和盲肠微生物群，如先前所示，该疫苗可诱导一定程度的保护作用。这些数据可能有助于改进未来针对家禽[疾病名称]的疫苗接种方案。在此，在19日龄时，用[细菌名称]对一个接种疫苗组和一个安慰剂组进行攻毒。在接种疫苗组中观察到[细菌名称]载量部分降低。这伴随着特异性全身和黏膜抗体的产生。分别在接种疫苗组和安慰剂组中观察到白细胞介素-10和抗菌肽禽β-防御素10基因表达的短暂相对较高水平。盲肠微生物群分析揭示了疫苗接种对其结构和组成的影响。具体而言，在第40天，分类为[细菌分类名称1]和[细菌分类名称2]的操作分类单元水平增加。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4e4/9231206/d3a6e657f366/vaccines-10-00981-g008a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4e4/9231206/95629e7691fc/vaccines-10-00981-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4e4/9231206/bdcc0040bbad/vaccines-10-00981-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4e4/9231206/3be5fabd8efe/vaccines-10-00981-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4e4/9231206/69ba87213926/vaccines-10-00981-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4e4/9231206/cb30b3fdca02/vaccines-10-00981-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4e4/9231206/e06afb8b65a9/vaccines-10-00981-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4e4/9231206/943e7f6f2785/vaccines-10-00981-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4e4/9231206/d3a6e657f366/vaccines-10-00981-g008a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4e4/9231206/95629e7691fc/vaccines-10-00981-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4e4/9231206/bdcc0040bbad/vaccines-10-00981-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4e4/9231206/3be5fabd8efe/vaccines-10-00981-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4e4/9231206/69ba87213926/vaccines-10-00981-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4e4/9231206/cb30b3fdca02/vaccines-10-00981-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4e4/9231206/e06afb8b65a9/vaccines-10-00981-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4e4/9231206/943e7f6f2785/vaccines-10-00981-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b4e4/9231206/d3a6e657f366/vaccines-10-00981-g008a.jpg

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