Department of Bacteriology, National Veterinary Institute, SE-75189 Uppsala, Sweden.
Int J Food Microbiol. 2010 Jun 30;141(1-2):51-5. doi: 10.1016/j.ijfoodmicro.2010.04.019. Epub 2010 Apr 28.
The proportions of broiler caeca and carcass rinse samples positive for Campylobacter spp. within broiler flocks were determined. Twenty intact caeca per flock from 27 flocks were analyzed individually. Rinse samples were obtained from 5 to 25 carcasses from 27 other flocks. In total, 540 caecum samples and 445 carcass rinse samples were analyzed. The proportion of positive caeca within flocks ranged from 10 to 100%, and the proportion of positive carcasses ranged from 85 to 100%. The highest and lowest numbers of Campylobacter spp. found in positive caecum samples were 8.6 and 1.7log cfu/g caecal contents, respectively. The number of Campylobacter spp. in the caeca from individual broilers within a flock varied by up to 6.3log cfu/g caecal contents. The highest number found on one carcass was 4.2log cfu/ml carcass rinse sample, and the within-flock variation in Campylobacter spp. was up to 3.2log cfu/ml rinse sample. There was thus great variation in the load of Campylobacter spp. in individual caecum and carcass samples obtained from each positive broiler flock. This large variation in the numbers of Campylobacter spp. carried by individual birds should be considered when only one or a few samples are collected from a flock and the results are used for risk assessments.
本研究测定了肉鸡盲肠和屠体冲洗样本中弯曲菌属阳性的比例。每个鸡群取 27 个鸡群中的 20 个完整盲肠进行个体分析。从其他 27 个鸡群中采集 5 至 25 个屠体的冲洗样本。总共分析了 540 个盲肠样本和 445 个屠体冲洗样本。鸡群中阳性盲肠的比例范围为 10%至 100%，阳性屠体的比例范围为 85%至 100%。在阳性盲肠样本中发现的最高和最低数量的弯曲菌分别为 8.6 和 1.7log cfu/g 盲肠内容物。鸡群中单个肉鸡盲肠内的弯曲菌数量变化可达 6.3log cfu/g 盲肠内容物。在一个屠体上发现的最高数量为 4.2log cfu/ml 屠体冲洗样本，鸡群内弯曲菌的变化高达 3.2log cfu/ml 冲洗样本。因此，从每个阳性肉鸡鸡群中获得的单个盲肠和屠体样本中，弯曲菌属的负荷存在很大差异。当从鸡群中仅采集一个或几个样本并将结果用于风险评估时，应考虑个体鸡只携带的弯曲菌数量的这种巨大差异。
