Agriculture Research Council, Fodder and Dairy Productions Research Centre, CRA-FLC, Via Lombardo 11, Lodi 26900, Italy.
Food Microbiol. 2011 Aug;28(5):1033-40. doi: 10.1016/j.fm.2011.02.009. Epub 2011 Feb 27.
Ninety-eight Lactobacillus plantarum strains isolated from Italian and Argentinean cheeses were evaluated for probiotic potential. After a preliminary subtractive screening based on the presence of msa and bsh genes, 27 strains were characterized. In general, the selected strains showed high resistance to lysozyme, good adaptation to simulated gastric juice, and a moderate to low bile tolerance. The capacity to agglutinate yeast cells in a mannose-specific manner, as well as the cell surface hydrophobicity was found to be variable among strains. Very high β-galactosidase activity was shown by a considerable number of the tested strains, whereas variable prebiotic utilization ability was observed. Only tetracycline resistance was observed in two highly resistant strains which harbored the tetM gene, whereas none of the strains showed β-glucuronidase activity or was capable of inhibiting pathogens. Three strains (Lp790, Lp813, and Lp998) were tested by in vivo trials. A considerable heterogeneity was found among a number of L. plantarum strains screened in this study, leading to the design of multiple cultures to cooperatively link strains showing the widest range of useful traits. Among the selected strains, Lp790, Lp813, and Lp998 showed the best probiotic potential and would be promising candidates for inclusion as starter cultures for the manufacture of probiotic fermented foods.
从意大利和阿根廷奶酪中分离出的 98 株植物乳杆菌菌株被评估为益生菌潜力。在基于 msa 和 bsh 基因存在的初步消减筛选后，对 27 株菌株进行了表征。一般来说，所选菌株对溶菌酶具有高抗性，对模拟胃液有良好的适应性，对胆汁的耐受性适中至较低。以甘露糖特异性方式凝集酵母细胞的能力以及细胞表面疏水性在菌株之间存在差异。大量测试菌株表现出非常高的β-半乳糖苷酶活性，而观察到可变的益生元利用能力。只有两种高度抗性的菌株表现出四环素抗性，这两种菌株都含有 tetM 基因，而没有任何菌株表现出β-葡萄糖醛酸酶活性或能够抑制病原体。对 3 株（Lp790、Lp813 和 Lp998）进行了体内试验。在本研究中筛选的许多植物乳杆菌菌株中发现了相当大的异质性，导致设计了多种培养物来共同连接显示最广泛有用特性的菌株。在所选择的菌株中，Lp790、Lp813 和 Lp998 表现出最佳的益生菌潜力，有望成为包含作为益生菌发酵食品制造起始培养物的候选物。
