NIZO Food Research, Health and Safety, Ede, the Netherlands.
Benef Microbes. 2010 Mar;1(1):61-6. doi: 10.3920/BM2008.1006.
Recently, we have identified the mannose-specific adhesin encoding gene (msa) of Lactobacillus plantarum. In the current study, structure and function of this potentially probiotic effector gene were further investigated, exploring genetic diversity of msa in L. plantarum in relation to mannose adhesion capacity. The results demonstrate that there is considerable variation in quantitative in vitro mannose adhesion capacity, which is paralleled by msa gene sequence variation. The msa genes of different L. plantarum strains encode proteins with variable domain composition. Construction of L. plantarum 299v mutant strains revealed that the msa gene product is the key-protein for mannose adhesion, also in a strain with high mannose adhering capacity. However, no straightforward correlation between adhesion capacity and domain composition of Msa in L. plantarum could be identified. Nevertheless, differences in Msa sequences in combination with variable genetic background of specific bacterial strains appears to determine mannose adhesion capacity and potentially affects probiotic properties. These findings exemplify the strain-specificity of probiotic characteristics and illustrate the need for careful and molecular selection of new candidate probiotics.
最近，我们已经确定了植物乳杆菌中甘露糖特异性黏附素编码基因（msa）。在本研究中，进一步研究了这个潜在益生菌效应基因的结构和功能，探讨了与甘露糖黏附能力相关的植物乳杆菌中 msa 的遗传多样性。结果表明，体外甘露糖黏附能力存在相当大的差异，与 msa 基因序列的变化相平行。不同植物乳杆菌菌株的 msa 基因编码具有可变结构域组成的蛋白质。植物乳杆菌 299v 突变株的构建表明，msa 基因产物是甘露糖黏附的关键蛋白，即使在具有高甘露糖黏附能力的菌株中也是如此。然而，在植物乳杆菌中，无法确定黏附能力与 Msa 的结构域组成之间存在直接的相关性。尽管如此，Msa 序列的差异以及特定细菌菌株的遗传背景的变化似乎决定了甘露糖的黏附能力，并可能影响益生菌的特性。这些发现体现了益生菌特性的菌株特异性，并说明了需要仔细和分子选择新的候选益生菌。
