采用“抑制剂阻断”技术测定线粒体中丙酮酸的初始转运速率。
Initial rates of pyruvate transport in mitochondria determined by an "inhibitor-stop" technique.
作者信息
Titheradge M A, Coore H G
出版信息
Biochem J. 1975 Sep;150(3):553-6. doi: 10.1042/bj1500553.
Abstract
An "inhibitor-stop" technique has been developed for measuring initial rates of pyruvate transport into mitochondria. The technique uses alpha-cyano-3-hydroxycinnamate as the inhibitor and separates the mitochondria from the radioactive medium by Millipore filtration. Observed rates depend on availability of hydroxyl and other exchangeable anions within the mitochondrial matrix.
摘要
一种“抑制剂阻断”技术已被开发出来用于测量丙酮酸转运到线粒体的初始速率。该技术使用α-氰基-3-羟基肉桂酸作为抑制剂，并通过微孔过滤将线粒体与放射性培养基分离。观察到的速率取决于线粒体内基质中羟基和其他可交换阴离子的可用性。
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