跨进化的脂质糖载体——多元醇和聚戊烯醇的液相色谱/串联质谱分析
Liquid chromatography/tandem mass spectrometry of dolichols and polyprenols, lipid sugar carriers across evolution.
作者信息
Guan Ziqiang, Eichler Jerry
机构信息
Department of Biochemistry, Duke University Medical Center, Durham, NC 27710, USA.
出版信息
Biochim Biophys Acta. 2011 Nov;1811(11):800-6. doi: 10.1016/j.bbalip.2011.04.009. Epub 2011 May 4.
Abstract
Across evolution, dolichols and polyprenols serve as sugar carriers in biosynthetic processes that include protein glycosylation and lipopolysaccharide biogenesis. Liquid chromatography coupled with electrospray ionization mass spectrometry offers a powerful tool for studying dolichols and polyprenols in their alcohol or glycan-modified forms in members of all three domains of life. In the following, recent examples of the how different versions of this analytical approach, namely reverse phase liquid chromatography-multiple reaction monitoring, normal phase liquid chromatography/tandem mass spectrometry and normal phase liquid chromatography-precursor ion scan detection have respectively served to address novel aspects of dolichol or polyprenol biology in Eukarya, Archaea and Bacteria.
摘要
在整个进化过程中，多萜醇和聚异戊二烯醇在包括蛋白质糖基化和脂多糖生物合成在内的生物合成过程中充当糖载体。液相色谱与电喷雾电离质谱联用为研究生命三域所有成员中醇形式或聚糖修饰形式的多萜醇和聚异戊二烯醇提供了一个强大的工具。以下是这种分析方法的不同版本，即反相液相色谱 - 多反应监测、正相液相色谱/串联质谱和正相液相色谱 - 前体离子扫描检测，分别如何用于解决真核生物、古细菌和细菌中多萜醇或聚异戊二烯醇生物学新问题的近期实例。
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