Guan Ziqiang, Chen Lingli, Gerritsen Jacoline, Smidt Hauke, Goldfine Howard
Department of Biochemistry, Duke University Medical Center, Durham, NC 27710, USA.
Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104-6076, USA.
Biochim Biophys Acta. 2016 Sep;1861(9 Pt A):1076-1082. doi: 10.1016/j.bbalip.2016.06.006. Epub 2016 Jun 14.
We have examined the lipids of three isolates, Romboutsia lituseburensis, Romboutsia ilealis, and Romboutsia sp. strain FRIFI, of the newly described genus Romboutsia by two-dimensional thin-layer chromatography (2D-TLC) and by liquid chromatography/mass spectrometry (LC/MS). We have found three phospholipids, phosphatidylglycerol (PG), cardiolipin and phosphatidic acid in all three species. A fourth phospholipid, lysyl-PG, was found in R. lituseburensis and strain FRIFI. Polyprenyl-phosphates were identified in the lipid extracts of all three species. Three glycolipids, mono-, di- and tri-hexosyldiacylglycerol, were common to all three species. An additional glycolipid, tetrahexosyl-diacylglycerol was identified in strain FRIFI. Acylated trihexosyldiacylglycerol and acyl-tetrahexosydiacylglycerol were also found in R. ilealis and strain FRIFI. Remarkably, no alk-1-enyl ether lipids (plasmalogens) were present in Romboutsia as distinct from bacteria of the related genus Clostridium in which these ether lipids are common. We have compared the lipidome of Romboutsia with that recently described for Clostridium difficile, which has plasmalogens, no lysyl-PG, and no tetrahexosyl-diacylglycerol. According to 16S rRNA gene sequencing, Romboutsia spp. and C. difficile are closely related (>95% sequence identity).
我们通过二维薄层色谱法（2D-TLC）和液相色谱/质谱法（LC/MS），对新描述的伦布茨菌属的三株分离菌，即利图塞布伦茨伦布茨菌、回肠伦布茨菌和伦布茨菌属菌株FRIFI的脂质进行了检测。我们在所有这三个物种中均发现了三种磷脂，即磷脂酰甘油（PG）、心磷脂和磷脂酸。在利图塞布伦茨伦布茨菌和菌株FRIFI中发现了第四种磷脂，即赖氨酰-PG。在所有这三个物种的脂质提取物中均鉴定出了聚异戊二烯磷酸酯。三种糖脂，即单、二和三己糖二酰甘油，在所有这三个物种中均常见。在菌株FRIFI中鉴定出了一种额外的糖脂，即四己糖二酰甘油。在回肠伦布茨菌和菌株FRIFI中还发现了酰化三己糖二酰甘油和酰基-四己糖二酰甘油。值得注意的是，与相关的梭菌属细菌不同，伦布茨菌中不存在alk-1-烯基醚脂（缩醛磷脂），而在梭菌属细菌中这些醚脂很常见。我们将伦布茨菌的脂质组与最近描述的艰难梭菌的脂质组进行了比较，艰难梭菌有缩醛磷脂，没有赖氨酰-PG，也没有四己糖二酰甘油。根据16S rRNA基因测序，伦布茨菌属物种与艰难梭菌密切相关（序列同一性>95%）。
