Institute of Legal Medicine and Traffic Medicine, University Hospital, Voss-Str. 2, 69115 Heidelberg, Germany.
Forensic Sci Int. 2011 Jul 15;210(1-3):e7-e11. doi: 10.1016/j.forsciint.2011.04.010. Epub 2011 May 13.
An LC/MS/MS procedure to determine THC along with its major metabolites 11-OH-THC, THC-COOH and its glucuronide as well as the cannabinoids CBD and CBN was applied to 5 post mortem cases to study their distribution into some less commonly studied matrices. Analytes were determined in fluids and tissue homogenates following protein precipitation and liquid-liquid extraction. Gall bladder fluid exhibited maximum concentrations of all analytes except THC, which was detectable in high concentrations in muscle tissue along with CBD. THC was also present in lung specimens, whereas its concentration in liver samples was low or not detectable at all. Liver und kidney specimens contained appreciable amounts of THC-COOglu. Findings from bile support extensive enterohepatic recirculation of the glucuronide. Muscle tissue seems an interesting specimen to detect multiple cannabis use, and brain may serve as an alternative specimen for blood; nevertheless, the present findings should be substantiated by further investigations.
一种 LC/MS/MS 方法被用于 5 个死后案例中，以研究大麻素及其主要代谢物 11-羟基-四氢大麻酚（11-OH-THC）、大麻酸（THC-COOH）及其葡糖苷酸以及大麻素 CBD 和 CBN 分布到一些研究较少的基质中。在进行蛋白质沉淀和液液萃取后，在液体和组织匀浆中检测分析物。除了 THC 之外，所有分析物在胆汁中均呈现出最大浓度，而 THC 则可在肌肉组织中以高浓度检测到，同时 CBD 也存在于肺标本中。THC 也存在于肝组织中，而在肝组织样本中 THC 的浓度很低或根本无法检测到。肝和肾组织中含有相当数量的 THC-COOglu。胆汁中的发现支持葡糖苷酸的广泛肠肝循环。肌肉组织似乎是检测多种大麻使用的有趣样本，而大脑可能是血液的替代样本；然而，目前的发现还需要进一步的研究来证实。
