GMCA Research Unit, Departamento de Microbiología y Ecología, Universidad de Valencia, Avda. Vicente Andrés Estellés s/n, 46100-Burjassot, Valencia, Spain.
Int J Med Microbiol. 2011 Aug;301(6):531-5. doi: 10.1016/j.ijmm.2011.02.001. Epub 2011 May 13.
Although Candida parapsilosis is the most prevalent among the 3 species of the *psilosis group, studies applying DNA-based diagnostic techniques with isolates previously identified as C. parapsilosis have revealed that both C. orthopsilosis and C. metapsilosis account for 0-10% of all these isolates, depending on the geographical area. Differences in the degrees of antifungal susceptibility and virulence have been found, so a more precise identification is required. In a first approach, we reidentified 38 randomly chosen clinical isolates, previously identified as C. parapsilosis, using the RPO2 (CA2) RAPD marker. Among them, we reclassified 4 as C. metapsilosis and 5 as C. orthopsilosis. We previously developed a method to identify different pathogen yeast species, including C. parapsilosis, based on the amplification of the RPS0 gene intron. In this work, we extend this approach to the new *psilosis species by partially sequencing their RPS0 gene, including the intron sequence. Based on intron sequences, we designed specific primers capable of identifying C. orthopsilosis and C. metapsilosis species, and we reidentified species among the initial isolates. These new primers have allowed a specific and rapid identification of C. orthopsilosis and C. metapsilosis.
虽然近平滑假丝酵母是假丝酵母组 3 个种中最常见的，但应用基于 DNA 的诊断技术对先前鉴定为近平滑假丝酵母的分离株进行的研究表明，根据地理位置的不同，近平滑假丝酵母和中间假丝酵母分别占所有这些分离株的 0-10%。已经发现它们在抗真菌药物敏感性和毒力方面存在差异，因此需要更精确的鉴定。在初步研究中，我们使用 RPO2 (CA2) RAPD 标记重新鉴定了 38 株先前鉴定为近平滑假丝酵母的随机临床分离株。其中，我们重新分类了 4 株为中间假丝酵母和 5 株为近平滑假丝酵母。我们之前开发了一种基于 RPS0 基因内含子扩增来鉴定不同病原体酵母种的方法，包括近平滑假丝酵母。在这项工作中，我们通过部分测序它们的 RPS0 基因，包括内含子序列，将这种方法扩展到新的假丝酵母种。基于内含子序列，我们设计了能够识别中间假丝酵母和近平滑假丝酵母种的特异性引物，并重新鉴定了最初分离株中的种。这些新的引物能够特异性和快速地鉴定中间假丝酵母和近平滑假丝酵母。
