Hyphal wall protein 1 gene: A potential marker for the identification of different species and phylogenetic analysis.

BACKGROUND AND PURPOSE

Hyphal wall protein 1 () is an important adhesin which usually is expressed on the germ tube and hyphal surface produced by different species. The hyphal wall protein-coding gene () was evaluated as a novel identification and phylogenetic marker in , , and

MATERIALS AND METHODS

Initially, four specific primer pairs were designed and the target was amplified and finally sequenced. A total of 77 isolates from four different species were included in the study. Consensus sequences were used for the evaluation of phylogenetic tree using the CLC Genome Workbench, GENEIOUS, and MEGA softwares and the levels of nucleotide and amino acid polymorphism were assessed.

RESULTS

According to the results, the specific amplified fragments of gene were useful for the differentiation of four species. Intra-species variation was observed only in with two DNA types. The phylogenetic tree of species based on the gene showed consistency in topology with those inferred from other gene sequences.

CONCLUSION

We found that gene was an excellent marker for the identification of non- species as well as the phylogenetic analysis of the most clinically significant species.