Department of Environmental Science and Engineering, Ewha Womans University, Seoul, Republic of Korea.
J Hazard Mater. 2011 Jul 15;191(1-3):393-6. doi: 10.1016/j.jhazmat.2011.04.080. Epub 2011 Apr 23.
The metabolism of cyclohexanes by Rodococcus sp. EC1 was investigated using a sequential tracking method of degradation intermediate. Evidence for the formation of cyclohexanol, cyclohexaone, 2-cyclohexen-1-one, and phenol was presented. EC1 metabolized cyclohexane to phenol by aromatization of 2-cyclohexen-1-one, and furthermore gamma-butyrolactone as an intermediate of 2-cyclohexen-1-one was formed. Aromatization by EC1 was confirmed using tetrahydrofuran. Tetrahydrofuran was metabolized through aromatization reaction, involving furan and 2,3-dihydrofuran as key intermediates. EC1 can degrade cyclohexane and tetrahydrofuran in aromatization via desaturation.
采用降解中间产物的连续跟踪方法研究了棒状杆菌 EC1 对环己烷的代谢。提出了环己醇、环己酮、2-环己烯-1-酮和苯酚形成的证据。EC1 通过 2-环己烯-1-酮的芳构化将环己烷代谢为苯酚，并且进一步形成 2-环己烯-1-酮的中间产物γ-丁内酯。使用四氢呋喃证实了 EC1 的芳构化。四氢呋喃通过涉及呋喃和 2,3-二氢呋喃作为关键中间体的芳构化反应进行代谢。EC1 可以通过去饱和作用在芳构化中降解环己烷和四氢呋喃。
