Salamanca Diego, Engesser Karl-Heinrich
Institute for Sanitary Engineering, Water Quality and Solid Waste Management, University of Stuttgart, Bandtäle 2, 70569, Stuttgart, Germany,
Environ Sci Pollut Res Int. 2014 Nov;21(22):12757-66. doi: 10.1007/s11356-014-3206-z. Epub 2014 Jun 28.
Two strains capable of degrading cyclohexane were isolated from the soil and sludge of the wastewater treatment plant of the University of Stuttgart and a biotrickling filter system. The strains were classified as gram negative and identified as Acidovorax sp. CHX100 and Chelatococcus sp. CHX1100. Both strains have demonstrated the capability to degrade cycloalkanes (C5-C8), while only strain CHX1100 used as well short linear n-alkanes (C5-C8) as the sole source of carbon and energy. The growth of Acidovorax sp. CHX100 using cyclohexane was much faster compared to Chelatococcus sp. CHX1100. Degenerated primers were optimized from a set sequences of cyclohexanol dehydrogenase genes (chnA) as well as cyclohexanone monooxygenases (chnB) and used to amplify the gene cluster, which encodes the conversion of cyclohexanol to caprolactone. Phylogenetic analysis has indicated that the two gene clusters belong to different groups. The cyclohexane monooxygenase-induced activity which oxidizes also indole to 5-hydroxyindole has indicated the presence of a CYP-type system monooxygenase involved in the transformation of cyclohexane to cyclohexanol.
从斯图加特大学污水处理厂的土壤和污泥以及一个生物滴滤系统中分离出了两株能够降解环己烷的菌株。这些菌株被归类为革兰氏阴性菌，并被鉴定为嗜酸菌属CHX100和螯球菌属CHX1100。两株菌株均已证明具有降解环烷烃（C5 - C8）的能力，而只有CHX1100菌株还能利用短链线性正构烷烃（C5 - C8）作为唯一的碳源和能源。与螯球菌属CHX1100相比，嗜酸菌属CHX100利用环己烷的生长速度要快得多。从一组环己醇脱氢酶基因（chnA）以及环己酮单加氧酶（chnB）的序列中优化出简并引物，并用于扩增编码环己醇转化为己内酯的基因簇。系统发育分析表明，这两个基因簇属于不同的组。将吲哚氧化为5 - 羟基吲哚的环己烷单加氧酶诱导活性表明，存在一种参与环己烷转化为环己醇的CYP型系统单加氧酶。
