一种促肿瘤佛波酯，佛波醇酯（TPA），通过激活病毒增强子的功能来增强多瘤病毒DNA的复制。
A tumor promoting phorbol ester, TPA, enhances polyomavirus DNA replication by activating the function of the viral enhancer.
作者信息
Murakami Y, Asano M, Satake M, Ito Y
机构信息
Department of Viral Oncology, Kyoto University, Japan.
出版信息
Oncogene. 1990 Jan;5(1):5-13.
The effect of tumor promoter, 12-O-tetradecanoylphorbol 13-acetate (TPA) on enhancer dependent polyomavirus (Py) DNA replication was examined in cells expressing Py large T antigen. The results showed that TPA enhanced Py DNA replication by stimulating the activity of the A element, one of the two cores of Py enhancer. Of the three subdomains of the A element, the biding sites of PEBP1 (PEA1), a member of AP1 family, and of PEBP5 were by themselves able to activate Py DNA replication. Furthermore, each binding site of PEBP1 and PEBP5 responded to TPA to enhance Py DNA replication. The results suggest that growth promoting signals could activate DNA replication directly via enhancer binding proteins.
在表达多瘤病毒（Py）大T抗原的细胞中，研究了肿瘤启动子12-O-十四酰佛波醇-13-乙酸酯（TPA）对增强子依赖性多瘤病毒（Py）DNA复制的影响。结果表明，TPA通过刺激Py增强子两个核心之一的A元件的活性来增强Py DNA复制。在A元件的三个亚结构域中，AP1家族成员PEBP1（PEA1）和PEBP5的结合位点自身能够激活Py DNA复制。此外，PEBP1和PEBP5的每个结合位点对TPA作出反应以增强Py DNA复制。这些结果表明，促生长信号可通过增强子结合蛋白直接激活DNA复制。
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