Extremophiles Lab., Department of Microbiology, Faculty of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, Tehran, Iran.
Microorganisms Bank, Iranian Biological Resource Centre (IBRC), ACECR, Tehran, Iran.
Int J Syst Evol Microbiol. 2013 Apr;63(Pt 4):1229-1234. doi: 10.1099/ijs.0.042689-0. Epub 2012 Jul 6.
A novel gram-positive, slightly halophilic bacterium, designated strain B48(T), was isolated from soil around the hypersaline lake Aran-Bidgol in Iran and characterized taxonomically using a polyphasic approach. Cells of strain B48(T) were non-motile rods and produced ellipsoidal endospores at a central or subterminal position in swollen sporangia. Strain B48(T) was a strictly aerobic bacterium, catalase- and oxidase-positive. The strain was able to grow at NaCl concentrations of 0.5-10.0 % (w/v), with optimum growth occurring at 2.5 % (w/v) NaCl. The optimum temperature and pH for growth were 35 °C and pH 7.5-8.0, respectively. On the basis of 16S rRNA gene sequence analysis, strain B48(T) was shown to belong to the genus Bacillus within the phylum Firmicutes and showed the closest phylogenetic similarity to the species Bacillus foraminis CV53(T) (97.4 %) and Bacillus purgationiresistens DS22(T) (96.9 %). The DNA G+C content of this new isolate was 40.1 mol%. The major cellular fatty acids of strain B48(T) were iso-C15 : 0 and anteiso-C15 : 0, and its polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an aminophospholipid and two unknown phospholipids. The only quinone present was menaquinone 7 (MK-7). The peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid. All these features confirm the placement of isolate B48(T) within the genus Bacillus. DNA-DNA hybridization experiments revealed a low level of relatedness between strain B48(T) and Bacillus foraminis IBRC-M 10625(T) (8.1 %). On the basis of polyphasic evidence from this study, a new species of the genus Bacillus, Bacillus persicus sp. nov., is proposed, with strain B48(T) ( = IBRC-M 10115(T) = DSM 25386(T) = CECT 8001(T)) as the type strain.
一株新的革兰氏阳性、轻度嗜盐细菌，命名为 B48(T) 菌株，从伊朗高盐湖泊 Aran-Bidgol 周围的土壤中分离得到，并采用多相分类法对其进行了分类学描述。B48(T) 菌株的细胞为非运动杆状，在膨胀的孢子囊中呈中央或亚末端位置产生椭圆形内生孢子。B48(T) 菌株是严格需氧菌，过氧化氢酶和氧化酶阳性。该菌株能够在 0.5-10.0%（w/v）的 NaCl 浓度下生长，最佳生长条件为 2.5%（w/v）NaCl。生长的最适温度和 pH 值分别为 35°C 和 pH7.5-8.0。基于 16S rRNA 基因序列分析，B48(T) 菌株属于厚壁菌门Firmicutes 中的芽孢杆菌属，与 Bacillus foraminis CV53(T)（97.4%）和 Bacillus purgationiresistens DS22(T)（96.9%）最为相似。该新分离株的 DNA G+C 含量为 40.1mol%。B48(T) 菌株的主要细胞脂肪酸为 iso-C15:0 和 anteiso-C15:0，其极性脂类图谱由二磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、氨基磷脂和两种未知磷脂组成。唯一存在的醌是menaquinone 7（MK-7）。肽聚糖含有 meso-二氨基庚二酸作为诊断二氨基酸。所有这些特征都证实了分离株 B48(T) 属于芽孢杆菌属。DNA-DNA 杂交实验表明，B48(T) 菌株与 Bacillus foraminis IBRC-M 10625(T)（8.1%）之间的亲缘关系较低。基于本研究的多相证据，提出了芽孢杆菌属的一个新种，即巴氏波斯菌（Bacillus persicus）sp. nov.，B48(T) 菌株（= IBRC-M 10115(T)=DSM 25386(T)=CECT 8001(T)）为模式菌株。
