Key Laboratory for Microbiological Engineering of Agricultural Environment of Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing 210095, PR China.
College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, PR China.
Int J Syst Evol Microbiol. 2012 Apr;62(Pt 4):800-805. doi: 10.1099/ijs.0.029827-0. Epub 2011 May 13.
A non-sporulating, non-motile, catalase- and oxidase-positive, Gram-negative, rod-shaped bacterial strain, designated BA-3T, was isolated from activated sludge of a wastewater treatment facility. The strain was able to degrade about 95 % of 100 mg 3-phenoxybenzoic acid l(-1) within 2 days of incubation. Growth occurred in the presence of 0-2 % (w/v) NaCl [optimum, 0.5 % (w/v) NaCl], at pH 5.5-9.0 (optimum, pH 7.0) and at 10-37 °C (optimum, 28 °C). Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain BA-3T was a member of the genus Sphingobium; it showed highest gene sequence similarity to Sphingobium qiguonii X23T (98.2 %), and similarities of <97.0 % with strains of other Sphingobium species. The polar lipid pattern, the presence of spermidine and ubiquinone Q-10, the predominance of summed feature 8 (C18:1ω6c and/or C18:1ω7c) in the cellular fatty acid profile and the DNA G+C content also supported affiliation of the isolate to the genus Sphingobium. Strain BA-3T showed low DNA-DNA relatedness values (21.3±0.8 %) with Sphingobium qiguonii X23(T). Based on phenotypic, genotypic and phylogenetic data, strain BA-3T represents a novel species of the genus Sphingobium, for which the name Sphingobium jiangsuense sp. nov. is proposed; the type strain is BA-3T (=CCTCC AB 2010217T= KCTC 23196T=KACC 16433T).
一株非产孢、非运动、接触酶和氧化酶阳性、革兰氏阴性、杆状的细菌菌株，被命名为 BA-3T，从废水处理设施的活性污泥中分离得到。该菌株在培养 2 天内能够降解约 100mg l(-1) 的 3-苯氧基苯甲酸的 95%。在 0-2%（w/v）NaCl（最佳，0.5%（w/v）NaCl）、pH 5.5-9.0（最佳，pH 7.0）和 10-37°C（最佳，28°C）的条件下能够生长。基于 16S rRNA 基因序列比较的系统发育分析表明，菌株 BA-3T 是鞘氨醇单胞菌属的一个成员；它与 Sphingobium qiguonii X23T（98.2%）的基因序列相似度最高，与其他 Sphingobium 种的菌株相似度均<97.0%。极性脂模式、腐胺和泛醌 Q-10 的存在、细胞脂肪酸图谱中特征 8（C18:1ω6c 和/或 C18:1ω7c）的优势以及 DNA G+C 含量也支持该分离株与鞘氨醇单胞菌属的归属。BA-3T 菌株与 Sphingobium qiguonii X23T 的 DNA-DNA 同源性值（21.3±0.8%）较低。基于表型、基因型和系统发育数据，BA-3T 株代表了鞘氨醇单胞菌属的一个新种，建议将其命名为 Sphingobium jiangsuense sp. nov.；其模式菌株为 BA-3T（=CCTCC AB 2010217T=KCTC 23196T=KACC 16433T）。
