sp. nov. and sp. nov., isolated from a wastewater treatment plant.

Two Gram-stain-negative, aerobic, motile and rod-shaped bacteria, one designated as strain AXB, capable of degrading estrogens, and another, YL23, capable of degrading estrogen and bisphenol A, were isolated from activated sludge in Xiamen City, PR China. The optimum temperature and pH of both strains were 25-35 °C and pH 7.0-8.0. While strain AXB could tolerate 3 % (w/v) NaCl, YL23 could only grow between 0-1 % (w/v) NaCl. They contained ubiquinone-10 as the major quinone, spermidine as the major polyamine, summed feature 8 (comprising Cω6 and/or Cω7) as the major fatty acids and diphosphatidylglycerol, phosphatidylcholine, phosphatidyldimethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol and sphingoglycolipid as the major polar lipids. The DNA G+C contents of strains AXB and YL23 were 63.6 and 63.7 mol%, respectively. Based on the results of 16S rRNA gene sequence analysis, strains AXB and YL23 belonged to the genus . Strain AXB was most closely related to NBRC 16172 (97.5 %) and DJ77 (97.2 %), and strain YL23 was most closely related to NBRC 16172 (97.4 %) and P25 (97.1 %). Average nucleotide identity values between these two strains and NBRC 16172, DJ77, IP26, P25 and UT26S were from 80.7 to 85.8 %. In conclusion, strains AXB and YL23 represent novel species of the genus , for which the names sp. nov. and sp. nov. are proposed, respectively. The type strains of and are AXB (=MCCC 1K01232=DSM 102173) and YL23 (=MCCC 1K02300=DSM 102172), respectively.