University of Chinese Academy of Sciences, Beijing 100049, PR China.
CAS Key Laboratory of Urban Pollutant Conversion, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, Fujian 361021, PR China.
Int J Syst Evol Microbiol. 2020 Mar;70(3):1822-1829. doi: 10.1099/ijsem.0.003978.
Two Gram-stain-negative, aerobic, motile and rod-shaped bacteria, one designated as strain AXB, capable of degrading estrogens, and another, YL23, capable of degrading estrogen and bisphenol A, were isolated from activated sludge in Xiamen City, PR China. The optimum temperature and pH of both strains were 25-35 °C and pH 7.0-8.0. While strain AXB could tolerate 3 % (w/v) NaCl, YL23 could only grow between 0-1 % (w/v) NaCl. They contained ubiquinone-10 as the major quinone, spermidine as the major polyamine, summed feature 8 (comprising Cω6 and/or Cω7) as the major fatty acids and diphosphatidylglycerol, phosphatidylcholine, phosphatidyldimethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol and sphingoglycolipid as the major polar lipids. The DNA G+C contents of strains AXB and YL23 were 63.6 and 63.7 mol%, respectively. Based on the results of 16S rRNA gene sequence analysis, strains AXB and YL23 belonged to the genus . Strain AXB was most closely related to NBRC 16172 (97.5 %) and DJ77 (97.2 %), and strain YL23 was most closely related to NBRC 16172 (97.4 %) and P25 (97.1 %). Average nucleotide identity values between these two strains and NBRC 16172, DJ77, IP26, P25 and UT26S were from 80.7 to 85.8 %. In conclusion, strains AXB and YL23 represent novel species of the genus , for which the names sp. nov. and sp. nov. are proposed, respectively. The type strains of and are AXB (=MCCC 1K01232=DSM 102173) and YL23 (=MCCC 1K02300=DSM 102172), respectively.
从中国福建省厦门市的活性污泥中分离到两株革兰氏阴性、需氧、能运动的杆状细菌，一株命名为 AXB 菌株，能够降解雌激素，另一株命名为 YL23 菌株，能够降解雌激素和双酚 A。两株菌的最适温度和 pH 值分别为 25-35°C 和 pH 7.0-8.0。AXB 菌株能够耐受 3%（w/v）NaCl，而 YL23 菌株只能在 0-1%（w/v）NaCl 下生长。它们都含有泛醌-10 作为主要的醌类物质，亚精胺作为主要的多胺类物质，summed feature 8（包含 Cω6 和/或 Cω7）作为主要的脂肪酸，二磷脂酰甘油、磷脂酰胆碱、磷脂酰二甲乙醇胺、磷脂乙醇胺、磷脂酰甘油和鞘糖脂作为主要的极性脂类。AXB 菌株和 YL23 菌株的 DNA G+C 含量分别为 63.6 和 63.7mol%。基于 16S rRNA 基因序列分析的结果，AXB 菌株和 YL23 菌株属于 属。AXB 菌株与 NBRC 16172（97.5%）和 DJ77（97.2%）最为密切相关，YL23 菌株与 NBRC 16172（97.4%）和 P25（97.1%）最为密切相关。这两株菌与 NBRC 16172、DJ77、IP26、P25 和 UT26S 的平均核苷酸同一性值在 80.7%至 85.8%之间。总之，AXB 菌株和 YL23 菌株代表了 属的新种，分别命名为 sp. nov. 和 sp. nov.。和 的模式菌株分别为 AXB（=MCCC 1K01232=DSM 102173）和 YL23（=MCCC 1K02300=DSM 102172）。
