Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
The Key Laboratory for Microbial Resources of the Ministry of Education, PR China, and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, PR China.
Int J Syst Evol Microbiol. 2010 Dec;60(Pt 12):2724-2728. doi: 10.1099/ijs.0.020362-0. Epub 2010 Jan 8.
A Gram-staining-negative, catalase-positive, carbaryl-degrading, non-spore-forming, non-motile, rod-shaped bacterium, designated strain X23(T), was isolated from a wastewater treatment system. Phylogenetic analysis based on 16S rRNA gene sequence indicated that the strain belongs to the genus Sphingobium. The highest 16S rRNA gene sequence similarity observed for the isolate was 96.6 % with the type strain of Sphingobium amiense. Chemotaxonomic data [major ubiquinone: Q-10; major polar lipids: diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, sphingoglycolipid, phosphatidylethanolamine and unknown aminolipids and phospholipids; major fatty acids: summed feature 7 (C(18 : 1)ω7c, C(18 : 1)ω9t and/or C(18 : 1)ω12t), C(16 : 1)ω5c, C(14 : 0) 2-OH and C(16 : 0) 2-OH] as well as the inability to reduce nitrate and the presence of spermidine as the major polyamine supported the affiliation of the strain to the genus Sphingobium. Based on the phylogenetic analysis, whole-cell fatty acid composition and biochemical characteristics, the strain could be separated from all recognized species of the genus Sphingobium. Strain X23(T) should be classified as a novel species of the genus Sphingobium, for which the name Sphingobium qiguonii sp. nov. is proposed, with strain X23(T) (=CCTCC AB 208221(T) =DSM 21541(T)) as the type strain.
一株革兰氏阴性、接触酶阳性、能分解杀铃脲、不产芽孢、不运动、杆状的细菌，被命名为 X23(T) 菌株，从一个废水处理系统中分离得到。基于 16S rRNA 基因序列的系统发育分析表明，该菌株属于鞘氨醇单胞菌属。该分离株与模式菌株鞘氨醇单胞菌属的 amiensense 的最高 16S rRNA 基因序列相似性为 96.6%。化学分类学数据[主要醌：Q-10；主要极性脂：双磷脂酰甘油、磷脂酰胆碱、磷脂酰甘油、神经鞘脂、磷脂酰乙醇胺和未知的氨基脂和磷脂；主要脂肪酸：求和特征 7（C(18：1)ω7c、C(18：1)ω9t 和/或 C(18：1)ω12t)、C(16：1)ω5c、C(14：0) 2-OH 和 C(16：0) 2-OH]以及不能还原硝酸盐和存在腐胺作为主要聚胺支持该菌株属于鞘氨醇单胞菌属。基于系统发育分析、全细胞脂肪酸组成和生化特性，该菌株可与鞘氨醇单胞菌属的所有公认种区分开来。X23(T) 菌株应被归类为鞘氨醇单胞菌属的一个新种，建议命名为棘孢鞘氨醇单胞菌新种，其模式菌株为 X23(T)=CCTCC AB 208221(T)=DSM 21541(T)。
