College of Marine Life Sciences, Ocean University of China, Qingdao, PR China.
Weifang Medical University, Weifang, Shandong Province, PR China.
Int J Syst Evol Microbiol. 2012 Apr;62(Pt 4):770-775. doi: 10.1099/ijs.0.029215-0. Epub 2011 May 13.
A novel bacterial strain, designated MY15T, was isolated from a saliva sample taken from a student during a teaching experiment in China. Phylogenetic analyses based on 16S rRNA gene sequences showed that the novel strain was most closely related to Myroides marinus JS-08T, Myroides odoratimimus LMG 4029T and Myroides profundi D25T with 96.5 %, 96.3 % and 96.1 % gene sequence similarities, respectively, demonstrating that the novel strain belonged to the genus Myroides. Strain MY15T formed pale yellow colonies that turned to brown on Luria-Bertani (LB) agar and that gave off a characteristic fruity odour. Cells were Gram-staining-negative, rod-shaped and non-motile. The new isolate contained menaquinone 6 (MK-6) as the major respiratory quinone and C15:0 iso (51.2 %), C17:0 iso 3-OH (12.9 %) and C13:0 iso (10.5 %) as the dominant fatty acids. The G+C content of the DNA was 34.3 mol%. On the basis of this study, based on a polyphasic taxonomic approach, strain MY15T (=DSM 23313T=LMG 25566T) represents a novel species of the genus Myroides, for which the name Myroides phaeus sp. nov. is proposed. Emended descriptions of the genus Myroides and of the species M. profundi and M. marinus are also given.
一株新的细菌菌株，命名为 MY15T，是从中国教学实验中采集的一名学生的唾液样本中分离得到的。基于 16S rRNA 基因序列的系统发育分析表明，该新菌株与 Myroides marinus JS-08T、Myroides odoratimimus LMG 4029T 和 Myroides profundi D25T 最为密切相关，其 16S rRNA 基因序列相似性分别为 96.5%、96.3%和 96.1%，表明该新菌株属于 Myroides 属。菌株 MY15T 在 Luria-Bertani（LB）琼脂上形成淡黄色菌落，随后变为棕色，并散发出特征性的水果气味。细胞革兰氏染色阴性，呈杆状，无动力。新分离株含有menaquinone 6（MK-6）作为主要呼吸醌和 C15:0 iso（51.2%）、C17:0 iso 3-OH（12.9%）和 C13:0 iso（10.5%）作为主要脂肪酸。DNA 的 G+C 含量为 34.3 mol%。基于这项研究，基于多相分类学方法，菌株 MY15T（=DSM 23313T=LMG 25566T）代表了 Myroides 属的一个新种，建议将其命名为 Myroides phaeus sp. nov.。对 Myroides 属和 M. profundi 和 M. marinus 种的描述也进行了修订。
