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通过聚合酶链反应和基因测序鉴定出菌血症的首例报告。

First report of bacteraemia identified by Polymerase chain reaction and genetic sequencing.

作者信息

Pérez-Lazo G, Morales-Moreno A, Soto-Febres F, Jove-Químper H, Morales-Castillo L, Palomares-Reyes C, Del Valle-Mendoza J, Aguilar-Luis M, Silva-Caso W

机构信息

Unidad de Infectología, Hospital Nacional Guillermo Almenara Irigoyen, EsSalud, Lima, Peru.

Servicio de Microbiología, Hospital Nacional Guillermo Almenara Irigoyen, EsSalud, Lima, Peru.

出版信息

IDCases. 2020 Jan 18;19:e00695. doi: 10.1016/j.idcr.2020.e00695. eCollection 2020.

DOI:10.1016/j.idcr.2020.e00695
PMID:32021799
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6994844/
Abstract

We report the first case of isolated from blood, causing bacteremia in an immunocompromised patient using the automated MicroScan Walk Away 96 system, followed by bacterial identification by amplification-sequencing of the 16S rDNA. The sequences obtained were compared with the reference sequence of the BLAST ® platform - National Library of Medicine, USA, and the isolation was identified as strain with 99.67 % identity in Blast report. In the literature we did not find previous reported cases of infections by this bacterium, however its pathogenic role is still controversial; therefore, this isolation alerts us to carry out an exhaustive surveillance of other possible acquisition routes.

摘要

我们报告了首例使用自动MicroScan Walk Away 96系统从血液中分离出的、导致免疫功能低下患者发生菌血症的病例,随后通过16S rDNA扩增测序进行细菌鉴定。将获得的序列与美国国立医学图书馆BLAST®平台的参考序列进行比较,在Blast报告中该分离株被鉴定为具有99.67%同一性的菌株。在文献中我们未发现此前有该细菌感染的报道病例,但其致病作用仍存在争议;因此,此次分离提醒我们要对其他可能的感染途径进行详尽监测。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c144/6994844/cdcdb49dcb7e/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c144/6994844/cdcdb49dcb7e/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c144/6994844/cdcdb49dcb7e/gr1.jpg

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