评价贝克曼库尔特 UniCel DxH 800 和雅培诊断 Cell-Dyn Sapphire 血液分析仪在三级医院儿科和新生儿标本中的性能。

Evaluation of the Beckman Coulter UniCel DxH 800 and Abbott Diagnostics Cell-Dyn Sapphire hematology analyzers on pediatric and neonatal specimens in a tertiary care hospital.

机构信息

Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA.

出版信息

Am J Clin Pathol. 2011 Jun;135(6):929-38. doi: 10.1309/AJCP2EXNSLGGRVSQ.

PMID:21571966

Abstract

We evaluated the new UniCel DxH 800 hematology analyzer (Beckman Coulter, Miami, FL) vs the Cell-Dyn Sapphire (Abbott Diagnostics, Santa Clara, CA) using 156 pediatric specimens in Microtainer tubes (Becton Dickinson, Franklin Lakes, NJ). The CBC and differential showed good interinstrument correlation, including WBCs (r = 0.995), RBCs (r = 0.992), hemoglobin (r = 0.998), mean corpuscular volume (r = 0.988), platelets (r = 0.997), neutrophils (r = 0.988), lymphocytes (r = 0.984), monocytes (r = 0.815), eosinophils (r = 0.840), basophils (r = 0.049), and nucleated RBCs (NRBCs; r = 0.906). In the instrument vs 400-cell manual differential comparison, the DxH 800 and Sapphire showed comparable performance for nearly all parameters except for NRBCs, for which the DxH 800 correlated better (r = 0.989) than the Sapphire (r = 0.906). We also compared clinical efficiency by determining whether flagged specimens showed abnormalities on a peripheral blood smear as defined by International Council for Standardization in Haematology criteria. The efficiency of the DxH 800 was 78.0% vs the Sapphire at 68.1%. Both instruments showed identical sensitivity (91.1%), but the specificity for the DxH 800 (71.9%) was higher than that of the Sapphire (57.3%).

摘要

我们使用 Microtainer 管（Becton Dickinson，富兰克林湖，新泽西州）评估了新的 UniCel DxH 800 血液分析仪（贝克曼库尔特，迈阿密，佛罗里达州）与 Cell-Dyn Sapphire（雅培诊断，圣克拉拉，加利福尼亚州）在 156 个儿科标本中的应用。CBC 和差异显示出良好的仪器间相关性，包括白细胞（r = 0.995）、红细胞（r = 0.992）、血红蛋白（r = 0.998）、平均红细胞体积（r = 0.988）、血小板（r = 0.997）、中性粒细胞（r = 0.988）、淋巴细胞（r = 0.984）、单核细胞（r = 0.815）、嗜酸性粒细胞（r = 0.840）、嗜碱性粒细胞（r = 0.049）和有核红细胞（NRBC；r = 0.906）。在仪器与 400 细胞手动差异比较中，DxH 800 和 Sapphire 在几乎所有参数上的性能都相当，除了 NRBC，DxH 800 的相关性更好（r = 0.989），而 Sapphire 的相关性稍差（r = 0.906）。我们还通过确定根据国际血液学标准化委员会标准定义的外周血涂片上标记标本是否显示异常来比较临床效率。DxH 800 的效率为 78.0%，而 Sapphire 的效率为 68.1%。两种仪器的敏感性均为 91.1%，但 DxH 800 的特异性（71.9%）高于 Sapphire（57.3%）。