Desai Ankur M, Andreae Mark, Mullen Douglas G, Holl Mark M Banaszak, Baker James R
Michigan Nanotechnology Institute for Medicine and Biological Sciences, University of Michigan, 9220 MSRB III, 1150 W. Medical Center Dr, Ann Arbor, MI, 48109-5648, USA.
Anal Methods. 2011 Jan;3(1):56-58. doi: 10.1039/c0ay00493f.
Acetonitrile is a choice of solvent for almost all chromatographic separations. In recent years, researchers around the globe have faced an acetonitrile shortage that affected routine analytical operations. Researchers have tried to counter this shortage by applying many innovative solutions, including using ultra performance liquid chromatography (UPLC) columns that are shorter and smaller in diameter than traditional high performance liquid chromatography (HPLC) columns, thus significantly decreasing the volume of eluent required. Although utilizing UPLC in place of HPLC can alleviate the solvent demand to some extent, acetonitrile is generally thought of as the solvent of choice due to its versatility. In the following communication, we describe an alternative eluent system that uses isopropanol in place of acetonitrile as an organic modifier for routine chromatographic separations. We report here the development of an isopropanol based UPLC protocol for G5 PAMAM dendrimer based conjugates that was transferred to semi-preparative applications.
乙腈几乎是所有色谱分离的首选溶剂。近年来，全球的研究人员都面临着乙腈短缺的问题，这影响了常规分析操作。研究人员尝试了许多创新解决方案来应对这一短缺，包括使用比传统高效液相色谱（HPLC）柱更短、直径更小的超高效液相色谱（UPLC）柱，从而显著减少所需洗脱液的体积。尽管用UPLC代替HPLC可以在一定程度上缓解溶剂需求，但由于乙腈的通用性，它通常仍被视为首选溶剂。在接下来的交流中，我们描述了一种替代洗脱液系统，该系统使用异丙醇代替乙腈作为常规色谱分离的有机改性剂。我们在此报告了一种基于异丙醇的UPLC方法的开发，该方法用于基于G5 PAMAM树枝状聚合物的缀合物，并已应用于半制备领域。
