Yang Gui-Ling, Yang Li-Wei, Li Yong-Xue, Cao Hui, Zhou Wen-Liang, Fang Zhi-Jian, Zhou Hong-Bo, Mo Jie-Li, Xiao Shu-Xiong, Lin Hui-Rong
School of Life Science, Sun Yat-Sen University, Guangzhou, 510275, China.
J Chromatogr Sci. 2010 Jan;48(1):18-21. doi: 10.1093/chromsci/48.1.18.
Ultra-performance liquid chromatography (UPLC), as a development of the chromatographic technique, was used in the quality control of Panax ginseng C.A. Mey. In the study, the UPLC methods were transferred from conventional high-performance liquid chromatography (HPLC) methods according to chromatographic equations. The results demonstrated that UPLC analysis methods could be transferred from HPLC without loss of efficiency. Compared with conventional HPLC, UPLC made a surprising 10-fold increase in speed and 20-fold decrease in solvent consumption. The study indicated UPLC as a suitable alternative to HPLC and can be reliably applied to quality control of traditional Chinese medicines.
超高效液相色谱法（UPLC）作为色谱技术的一项发展成果，被用于人参的质量控制。在该研究中，根据色谱方程，将UPLC方法从传统高效液相色谱法（HPLC）方法进行了转换。结果表明，UPLC分析方法可以从HPLC转换而来且不会损失效率。与传统HPLC相比，UPLC的速度惊人地提高了10倍，溶剂消耗量减少了20倍。该研究表明UPLC是HPLC的合适替代方法，可可靠地应用于中药的质量控制。
