Department of Pharmaceutical Sciences, Kumaun University, Nainital, Uttarakhand, India.
Indian J Pharmacol. 2011 Apr;43(2):192-6. doi: 10.4103/0253-7613.77365.
Gram-negative infections and control infusion of recombinant cytokines in human have been shown to induce sickness behavior characterized by fever, prolong sleep, decreased food and water intake, reduced mobility, depression, and anxiety. Therefore, the present study was undertaken to investigate the effect of bioflavonoid quercetin in lipopolysaccharide (LPS)-induced sickness behavior.
Wistar albino rats were divided into six groups (n=6). Three groups received vehicle and two doses of quercetin (2 and 25 mg/kg, i.p.) respectively for 2 weeks before being challenged with LPS (1 mg/kg, i.p). One group received vehicle for 2 weeks and was challenged with saline on day 15. The per se effect of quercetin (2 and 25 mg/kg, i.p.) was also seen after 2 weeks of dosing. LPS-induced sickness behavior in rats was quantified by measuring time in social exploration, anxiety, food and water consumption, and weight loss. Levels of cytokines (TNF-α, IL-1β, and IL-6) and oxidative stress in rat brain were also analyzed.
Quercetin (2 and 25 mg/kg) administration significantly (P<0.05) attenuated LPS-induced sickness behavior by modulating cytokines production as well inhibiting LPS-induced oxidative stress.
Adequate intake of dietary flavonoids (like quercetin) may help promote recovery from sickness behavior.
革兰氏阴性感染和控制输注重组细胞因子在人类中已被证明会引起疾病行为，其特征为发热、延长睡眠时间、减少食物和水的摄入、运动减少、抑郁和焦虑。因此，本研究旨在探讨生物类黄酮槲皮素对脂多糖（LPS）诱导的疾病行为的影响。
Wistar 白化大鼠被分为六组（n=6）。三组分别接受载体和两种剂量的槲皮素（2 和 25mg/kg，腹腔注射），连续 2 周，然后用 LPS（1mg/kg，腹腔注射）进行攻毒。一组接受载体连续 2 周，在第 15 天用生理盐水攻毒。还观察了槲皮素（2 和 25mg/kg，腹腔注射）连续 2 周给药后的自身作用。通过测量社交探索、焦虑、食物和水的消耗以及体重减轻来量化 LPS 诱导的大鼠疾病行为。还分析了大鼠大脑中的细胞因子（TNF-α、IL-1β 和 IL-6）和氧化应激水平。
槲皮素（2 和 25mg/kg）的给药显著（P<0.05）减轻了 LPS 诱导的疾病行为，通过调节细胞因子的产生以及抑制 LPS 诱导的氧化应激。
适当摄入膳食类黄酮（如槲皮素）可能有助于促进疾病行为的恢复。
