Ramanujan V Krishnan, Ren Songyang, Park Sangyong, Farkas Daniel L
Departments of Surgery and Biomedical Sciences, Principal Investigator, Metabolic Photonics Laboratory Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.
J Cell Sci Ther. 2010 Oct 2;1(102). doi: 10.4172/2157-7013.1000102.
We report here a non-invasive multispectral imaging platform for monitoring spectral reflectance and fluorescence images from primary breast carcinoma and metastatic lymph nodes in preclinical rat model in vivo. The system is built around a monochromator light source and an acousto-optic tunable filter (AOTF) for spectral selection. Quantitative analysis of the measured reflectance profiles in the presence of a widely-used lymphazurin dye clearly demonstrates the capability of the proposed imaging platform to detect tumor-associated spectral signatures in the primary tumors as well as metastatic lymphatics. Tumor-associated changes in vascular oxygenation and interstitial fluid pressure are reasoned to be the physiological sources of the measured reflectance profiles. We also discuss the translational potential of our imaging platform in intra-operative clinical setting.
我们在此报告一种非侵入性多光谱成像平台，用于在体内临床前大鼠模型中监测原发性乳腺癌和转移性淋巴结的光谱反射率和荧光图像。该系统围绕单色仪光源和声光可调滤光器（AOTF）构建，用于光谱选择。在存在广泛使用的淋巴蓝染料的情况下，对测量的反射率曲线进行定量分析，清楚地证明了所提出的成像平台能够检测原发性肿瘤以及转移性淋巴管中与肿瘤相关的光谱特征。血管氧合和间质液压力中与肿瘤相关的变化被认为是测量反射率曲线的生理来源。我们还讨论了我们的成像平台在术中临床环境中的转化潜力。
