Ricordi C, Mazzeferro V, Casavilla A, Scotti C, Pinna A, Tzakis A, Starzl T E
University of Pittsburgh Trasplant Institute, Pittsburgh, Pennsylvania 15213, U.S.A.
Diabetes Nutr Metab. 1992;5(S1):39-41.
The outcome of human islet isolation procedures can be significantly effected by the technique used for pancreas procurement. In fact, the final step of islet purification using discontinuous density gradients requires a significant difference between the density of the islets and the density of the non-endocrine component of the gland. Therefore, any procedure during multi-organ procurement that will result in edema or degranulation of the acinar tissue will result in failure of the islet purification step. In this report a technique for combined harvesting of liver and pancreas is presented. The use of this procedure can be of assistance to avoid damage to the pancreas that could result in a compromised islet purification for improper handling of the gland even before it arrives to the isolation facility.
人类胰岛分离程序的结果会受到胰腺获取所用技术的显著影响。事实上，使用不连续密度梯度进行胰岛纯化的最后一步要求胰岛密度与腺体非内分泌成分的密度之间存在显著差异。因此，多器官获取过程中任何导致腺泡组织水肿或脱颗粒的程序都会导致胰岛纯化步骤失败。在本报告中，提出了一种肝脏和胰腺联合获取的技术。使用该程序有助于避免对胰腺造成损伤，否则即使在胰腺到达分离设施之前，因处理不当也可能导致胰岛纯化受损。
