Department of Pharmacology, School of Pharmacy, Fudan University, Shanghai, China.
PLoS One. 2011 May 10;6(5):e19766. doi: 10.1371/journal.pone.0019766.
Hydrogen sulfide (H(2)S), the third physiologically relevant gaseous molecule, is recognized increasingly as an anti-inflammatory mediator in various inflammatory conditions. Herein, we explored the effects and mechanisms of sodium hydrosulfide (NaHS, a H(2)S donor) on tumor necrosis factor (TNF)-α-induced human umbilical vein endothelial cells (HUVEC) dysfunction.
Application of NaHS concentration-dependently suppressed TNF-α-induced mRNA and proteins expressions of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1), mRNA expression of P-selectin and E-selectin as well as U937 monocytes adhesion to HUVEC. Western blot analysis revealed that the expression of the cytoprotective enzyme, heme oxygenase-1 (HO-1), was induced and coincident with the anti-inflammatory action of NaHS. Furthermore, TNF-α-induced NF-κB activation assessed by IκBα degradation and p65 phosphorylation and nuclear translocation and ROS production were diminished in cells subjected to treatment with NaHS.
H(2)S can exert an anti-inflammatory effect in endothelial cells through a mechanism that involves the up-regulation of HO-1.
硫化氢（H₂S），第三种具有生理相关性的气体分子，在各种炎症情况下，作为一种抗炎介质，其作用正得到越来越多的认可。在此，我们研究了氢硫化钠（NaHS，一种 H₂S 供体）对肿瘤坏死因子（TNF）-α诱导的人脐静脉内皮细胞（HUVEC）功能障碍的影响及其作用机制。
NaHS 的浓度依赖性抑制 TNF-α诱导的细胞间黏附分子-1（ICAM-1）和血管细胞黏附分子-1（VCAM-1）mRNA 和蛋白表达、P-选择素和 E-选择素 mRNA 表达以及 U937 单核细胞黏附到 HUVEC。Western blot 分析显示，保护酶血红素加氧酶-1（HO-1）的表达被诱导，且与 NaHS 的抗炎作用一致。此外，NaHS 处理的细胞中 TNF-α诱导的 NF-κB 激活，表现为 IκBα 降解和 p65 磷酸化以及核转位和 ROS 产生减少。
H₂S 通过上调 HO-1 在血管内皮细胞中发挥抗炎作用。
