Laboratório de Imunopatologia, Núcleo de Pesquisas em Ciências Biológicas, Departamento de Análises Clínicas, Universidade Federal de Ouro Preto, Ouro Preto, Minas Gerais, Brazil.
PLoS One. 2011 May 10;6(5):e18873. doi: 10.1371/journal.pone.0018873.
The bone marrow is considered to be an important storage of parasites in Leishmania-infected dogs, although little is known about cellular genesis in this organ during canine visceral leishmaniasis (CVL).
METHODOLOGY/PRINCIPAL FINDINGS: The aim of the present study was to evaluate changes in erythropoiesis and leucopoiesis in bone marrow aspirates from dogs naturally infected with Leishmania chagasi and presenting different clinical statuses and bone marrow parasite densities. The evolution of CVL from asymptomatic to symptomatic status was accompanied by increasing parasite density in the bone marrow. The impact of bone marrow parasite density on cellularity was similar in dogs at different clinical stages, with animals in the high parasite density group. Erythroid and eosinophilic hypoplasia, proliferation of neutrophilic precursor cells and significant increases in lymphocytes and plasma cell numbers were the major alterations observed. Differential bone marrow cell counts revealed increases in the myeloid:erythroid ratio associated to increased numbers of granulopoietic cells in the different clinical groups compared with non-infected dogs.
Analysis of the data obtained indicated that the assessment of bone marrow constitutes an additional and useful tool by which to elaborate a prognosis for CVL.
骨髓被认为是利什曼原虫感染犬体内寄生虫的重要储存库，尽管人们对犬内脏利什曼病（CVL）期间该器官中的细胞发生知之甚少。
方法/主要发现：本研究旨在评估自然感染利什曼原虫且具有不同临床状态和骨髓寄生虫密度的犬骨髓抽吸物中的红细胞生成和白细胞生成的变化。从无症状到有症状的 CVL 演变伴随着骨髓中寄生虫密度的增加。在不同临床阶段的犬中，骨髓寄生虫密度对细胞密度的影响相似，高寄生虫密度组的动物。观察到的主要变化是红细胞和嗜酸性粒细胞发育不全、中性粒细胞前体细胞增殖以及淋巴细胞和浆细胞数量显著增加。与未感染的犬相比，不同临床组的骨髓细胞计数差异分析显示骨髓粒细胞：红细胞比值增加，粒状细胞数量增加。
对所获得数据的分析表明，骨髓评估是制定 CVL 预后的另一个有用工具。
