Departamento de Patologia Geral, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
Instituto Federal de Educação, Ciência e Tecnologia de Minas Gerais, Campus Bambuí, Minas Gerais, Brazil.
Biomed Res Int. 2021 Oct 5;2021:3192960. doi: 10.1155/2021/3192960. eCollection 2021.
Dogs are the main reservoir of in endemic regions. Canine leishmaniasis, caused by , can progress to a chronic disease resulting in death. Vaccines have been developed with a certain degree of success. The pathogenesis of this disease is not completely understood, especially in previously vaccinated dogs. We herein described clinical data, parasite load, serum levels of cytokines, and the reservoir potential in vdogs vaccinated with the fucose-mannose ligand (FML)/QuilA saponin vaccine (Leishmune™) naturally infected (Vi) and compared to vaccinated not infected dogs (Vn). Thirty-four dogs from private owners were divided into two groups: vaccinated/infected and vaccinated/uninfected. Clinical evaluation, hematological and biochemical parameters, and serum levels of cytokines were measured by conventional methods. The parasite burden in the bone marrow was measured by quantitative real-time PCR, and the transmissibility of parasites to sand flies was assessed by xenodiagnosis. Clinical, biochemical, and hematological parameters of vaccinated infected dogs were mostly normal. Vi dogs developed mild disease with low clinical scores. Serum levels of IL-10 were higher in Vi dogs, and a strong correlation was observed in IL-4 levels and the A/G ratio in Vi dogs. These results suggest a role of TH2 response in Vi dogs, although more data is needed to better understand the disease in vaccinated dogs.
在流行地区，狗是 的主要宿主。由 引起的犬利什曼病可进展为慢性疾病，导致死亡。已经开发出具有一定成功度的疫苗。这种疾病的发病机制尚不完全清楚，特别是在以前接种过疫苗的狗中。我们在此描述了临床数据、寄生虫载量、细胞因子血清水平，以及用岩藻糖甘露聚糖（FML）/QuilA 皂苷疫苗（Leishmune™）自然感染（Vi）和接种但未感染（Vn）的 vdogs 的储库潜力。从私人所有者那里招募了 34 只狗，分为两组：接种/感染和接种/未感染。通过常规方法测量临床评估、血液学和生化参数以及细胞因子的血清水平。通过实时定量 PCR 测量骨髓中的寄生虫负担，并通过异种诊断评估寄生虫对沙蝇的传染性。接种感染狗的临床、生化和血液学参数大多正常。Vi 狗的病情较轻，临床评分较低。Vi 狗的血清 IL-10 水平较高，并且在 Vi 狗中观察到 IL-4 水平和 A/G 比值之间存在很强的相关性。这些结果表明，TH2 反应在 Vi 狗中起作用，尽管需要更多的数据来更好地了解接种疫苗的狗中的疾病。
