Hsu T, Savaraji N, Trizna Z, Feun L, Furlong C, Schantz S, Shirley L
UNIV MIAMI,SCH MED,DEPT ONCOL,MIAMI,FL 33152. UNIV TEXAS,MD ANDERSON CANC CTR,DEPT HEAD & NECK SURG,HOUSTON,TX 77030. UNIV MIAMI,SYLVESTER COMPREHENS CANC CTR,DEPT MED,MIAMI,FL 33152. MEM SLOAN KETTERING CANC CTR,DEPT HEAD & NECK SURG,NEW YORK,NY 10021.
Int J Oncol. 1993 Nov;3(5):823-6. doi: 10.3892/ijo.3.5.823.
Using the UV-mimetic mutagen 4-nitroquinoline-1-oxide (4NQO) to induce genetic damage in human cells (lymphoblastoid lines and primary cultures of peripheral blood samples), chromosome aberrations were induced by treating the cells with 4NQO at 1 X 10(-5) M for 24 h. The overwhelming majority of chromosome aberrations was of the chromatid (S + G2) type instead of the chromosome (G1) type. The most common chromatid aberrations were simple breaks, isochromatid breaks, and chromatid exchanges. When the number of chromatid breaks per cell value was used as a measurement for 4NQO sensitivity, lymphoblastoid cells from a xeroderma pigmentosum patient showed the highest sensitivity, followed by the cells of two melanoma patients and normal persons. These preliminary results suggest that 4NQO may be employed to develop an assay system as a biomarker for determining UV sensitivity in the human population.
使用紫外线模拟诱变剂4-硝基喹啉-1-氧化物（4NQO）在人类细胞（淋巴母细胞系和外周血样本原代培养物）中诱导遗传损伤，通过用1×10⁻⁵ M的4NQO处理细胞24小时来诱导染色体畸变。绝大多数染色体畸变是染色单体（S + G2）型而非染色体（G1）型。最常见的染色单体畸变是简单断裂、等染色单体断裂和染色单体交换。当将每个细胞的染色单体断裂数作为4NQO敏感性的衡量指标时，一名着色性干皮病患者的淋巴母细胞显示出最高的敏感性，其次是两名黑色素瘤患者和正常人的细胞。这些初步结果表明，4NQO可用于开发一种检测系统，作为确定人群紫外线敏感性的生物标志物。
